FastQCFastQC Report
Thu 26 May 2016
SRR1512405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104508
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67450.3205024642339207No Hit
GTCCTACAGTGGACATTTCTAAATT51520.2448078125623661No Hit
GTCCTAAAGTGTGTATTTCTCATTT49330.23440157984669102No Hit
CTGTAGGACGTGGAATATGGCAAGA48150.228794568611761No Hit
GGTATCAACGCAGAGTACTTTTTTT44160.20983526791059953No Hit
CTTTAGGACGTGAAATATGGCGAGG43750.2078870690916832No Hit
TATCAACGCAGAGTACTTTTTTTTT40880.19424967735926876No Hit
GTCCTACAGTGTGCATTTCTCATTT23720.11271042923096514No Hit
CTGAAGGACCTGGAATATGGCGAGA23250.11047712814586591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.014.2739311
TATTCCG400.00534511414.22365955
TAGGACC8800.011.7452944
GTTTAGG2900.011.5787791
AATGGCG600.00592552711.07104616
AACGGGG1201.2956298E-711.07104616
GGACCGT600.005958292611.0628466
ACGTTAA600.00610712411.0262288
CCACCTT7650.010.91181513
TTAGGAC19400.010.8512983
TATAGCG700.001505642710.84510616
AGGACGT33000.010.8329685
AGGACCT16550.010.7142975
ACCTTTT8250.010.69824115
CACCTTT8000.010.67667214
TAGAAAT8350.010.6748424
GGACGTG32650.010.65866856
GTGGTAT3800.010.6557031
GACGTGG16100.010.542847
GACGTGA16900.010.4926547
Produced by FastQC (version 0.11.5)