##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512405_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104508 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.851880344479564 31.0 24.0 33.0 10.0 34.0 2 27.574245381818457 31.0 26.0 34.0 16.0 34.0 3 28.736141654011295 31.0 28.0 34.0 16.0 34.0 4 32.54249306726323 35.0 33.0 37.0 24.0 37.0 5 32.83776968298529 35.0 35.0 37.0 26.0 37.0 6 33.088525679161116 37.0 35.0 37.0 27.0 37.0 7 32.97641871639357 37.0 35.0 37.0 26.0 37.0 8 32.99137375576619 37.0 35.0 37.0 26.0 37.0 9 34.42263369870773 39.0 35.0 39.0 26.0 39.0 10 34.341337737846565 39.0 35.0 39.0 26.0 39.0 11 34.39016720297571 39.0 35.0 39.0 26.0 39.0 12 34.292605207487924 39.0 35.0 39.0 26.0 39.0 13 34.24154339161457 39.0 35.0 39.0 25.0 39.0 14 35.270567277482435 40.0 36.0 41.0 25.0 41.0 15 35.20564949147259 40.0 36.0 41.0 25.0 41.0 16 35.05722382618645 40.0 36.0 41.0 23.0 41.0 17 34.997375158469346 40.0 36.0 41.0 22.0 41.0 18 34.95689348769404 40.0 36.0 41.0 22.0 41.0 19 34.963093511642626 40.0 36.0 41.0 21.0 41.0 20 34.86994252338314 40.0 35.0 41.0 19.0 41.0 21 34.74879401741405 40.0 35.0 41.0 18.0 41.0 22 34.628329756883794 40.0 35.0 41.0 18.0 41.0 23 34.44953309752208 39.0 35.0 41.0 16.0 41.0 24 34.31709264113037 39.0 34.0 41.0 10.0 41.0 25 34.16770237984365 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.004140280178326 1101 2 2.1376155855339434 1101 3 0.8706778150583645 1101 4 0.5842679990570687 1101 5 0.09970070154286503 1101 6 -0.005879486277784451 1101 7 -0.3379570532195615 1101 8 -0.3518794639859095 1101 9 -0.5419149832319263 1101 10 -0.6898151573886651 1101 11 -0.47969173240833385 1101 12 -0.6073423591187321 1101 13 -0.6398622953630522 1101 14 -0.6982792625190442 1101 15 -0.7667235629599389 1101 16 -1.0000355785765294 1101 17 -0.996214809426192 1101 18 -0.9495670272200556 1101 19 -0.8758914680920853 1101 20 -0.9666848088487185 1101 21 -1.0641258986784692 1101 22 -0.9593760104334237 1101 23 -0.9059561695898495 1101 24 -1.0751758830980336 1101 25 -1.2670307203041986 1102 1 3.360601529545935 1102 2 1.7797450032505822 1102 3 0.7376139012083556 1102 4 0.49887687905899725 1102 5 0.33578323786029074 1102 6 0.09407112684225893 1102 7 0.009436283165321413 1102 8 -0.0778629856318176 1102 9 -0.20398826854354013 1102 10 -0.1435360371978618 1102 11 -0.32037808958389036 1102 12 -0.3696216238565029 1102 13 -0.29446250254703443 1102 14 -0.31263157165682287 1102 15 -0.5267141674071638 1102 16 -0.49873345132701985 1102 17 -0.5027049681869471 1102 18 -0.7104911641542131 1102 19 -0.5116258871337394 1102 20 -0.6154417049435423 1102 21 -0.6829025495252736 1102 22 -0.6076992656310907 1102 23 -0.804793771073868 1102 24 -0.7322020701899916 1102 25 -0.8771779172976224 1103 1 2.573769519024328 1103 2 1.0124071884929364 1103 3 -0.09868974573334555 1103 4 -0.32518672550401817 1103 5 -0.664482085596859 1103 6 -0.6011370675882262 1103 7 -0.6614653599221114 1103 8 -0.7672906906980828 1103 9 -0.9521447043498767 1103 10 -1.1615807722854044 1103 11 -1.2477730790348858 1103 12 -1.2950164123740677 1103 13 -1.1485994478109376 1103 14 -1.3985593817564634 1103 15 -1.2827312102151183 1103 16 -1.4635586849121296 1103 17 -1.5184060888474704 1103 18 -1.5057801886224311 1103 19 -1.5121309607027698 1103 20 -1.3493204507536234 1103 21 -1.6870847048102817 1103 22 -1.756685465157986 1103 23 -1.5289226790006865 1103 24 -1.6104702067151209 1103 25 -2.0441526818858407 1104 1 3.5497391025389575 1104 2 1.4104988059874906 1104 3 0.30750172712554047 1104 4 0.07586166811137929 1104 5 -0.13182383336670256 1104 6 -0.3483393735038476 1104 7 -0.39788964289208195 1104 8 -0.45413426232197196 1104 9 -0.621141851192057 1104 10 -0.6533033469722724 1104 11 -0.6738498064508249 1104 12 -0.5042251985179362 1104 13 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-0.6160383336379027 1105 25 -0.51397928176889 1106 1 2.285019600766873 1106 2 0.9130742931144162 1106 3 0.20368029644794206 1106 4 -0.0021367819865076854 1106 5 0.01075511736784307 1106 6 0.026672151089847773 1106 7 0.06274192976020743 1106 8 -0.09867986939603668 1106 9 -0.32955696414968827 1106 10 -0.2801901617573108 1106 11 -0.3232313822178625 1106 12 -0.16761216654892053 1106 13 -0.33244259103285145 1106 14 -0.5700368552216588 1106 15 -0.28376127309169163 1106 16 -0.4251360573798735 1106 17 -0.5021037410307088 1106 18 -0.4925090465818869 1106 19 -0.3797533490552851 1106 20 -0.36478357514376114 1106 21 -0.40709587735948816 1106 22 -0.265747240293976 1106 23 -0.42697808270626325 1106 24 -0.4007062364339333 1106 25 -0.5160084576962944 1107 1 3.955329887534919 1107 2 2.107678350093863 1107 3 0.9285379814574313 1107 4 0.529553358858557 1107 5 0.13794329386515614 1107 6 0.041237722888759265 1107 7 -0.20324679194383322 1107 8 -0.08734144033972768 1107 9 -0.35775938550896313 1107 10 -0.4297144421646095 1107 11 -0.15749789209305476 1107 12 -0.29286272110968525 1107 13 -0.23530091746201265 1107 14 -0.4441849697150744 1107 15 -0.3473687242502592 1107 16 -0.5893729015950697 1107 17 -0.39812693904230656 1107 18 -0.35635435753680156 1107 19 -0.36498065215280917 1107 20 -0.41313058318822726 1107 21 -0.4113452827099948 1107 22 -0.4520997004056895 1107 23 -0.5252088334345473 1107 24 -0.5719719712603464 1107 25 -0.4577328075907232 1108 1 3.655487023181223 1108 2 1.8033599330948356 1108 3 0.5088496270272564 1108 4 0.23282283222174271 1108 5 0.006600073571092935 1108 6 -0.19936410582115371 1108 7 -0.42747006318670344 1108 8 -0.47924544815492 1108 9 -0.5625590355213177 1108 10 -0.7303297338196231 1108 11 -0.7068123501218508 1108 12 -0.5310985452645411 1108 13 -0.6708477813133911 1108 14 -0.6762434140640181 1108 15 -0.8037780564930728 1108 16 -0.8580224646405483 1108 17 -0.776660350549335 1108 18 -0.7772532354072581 1108 19 -0.7592245703835587 1108 20 -0.6933049898931216 1108 21 -0.7019345128431809 1108 22 -0.639329432394419 1108 23 -0.7203371391339104 1108 24 -0.89813220232395 1108 25 -0.8420754269041382 1109 1 3.421398997691714 1109 2 1.8052465114840537 1109 3 0.5691302115582992 1109 4 0.19084368143920472 1109 5 -0.13844739823511532 1109 6 -0.3377196325491667 1109 7 -0.5138679843936487 1109 8 -0.46462597045058374 1109 9 -0.45473331939491146 1109 10 -0.6343234504538344 1109 11 -0.36777453246057235 1109 12 -0.5269583967313736 1109 13 -0.5696912100816078 1109 14 -0.7828946471344267 1109 15 -0.7938882488682566 1109 16 -0.9245621555649066 1109 17 -0.9078642804984156 1109 18 -0.9193724340373493 1109 19 -0.9234296684316448 1109 20 -0.7591557504707609 1109 21 -0.8298761925272373 1109 22 -0.9831842904386505 1109 23 -0.9157511284510207 1109 24 -1.1946874411494903 1109 25 -1.1715298714077633 1110 1 2.285571327523062 1110 2 0.40247676519691566 1110 3 -0.7031932202963667 1110 4 -0.9383716946118916 1110 5 -0.8997471272975943 1110 6 -0.8893997689894206 1110 7 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2.1301266000859016 1201 17 1.901041830168957 1201 18 2.081184794472847 1201 19 1.9852174900700916 1201 20 2.161117128328364 1201 21 2.1363880267939876 1201 22 2.119656188010964 1201 23 2.1686131048213113 1201 24 2.1701091786028286 1201 25 2.1047818387942527 1202 1 5.487293293012382 1202 2 3.8467284829566886 1202 3 2.6269429175288437 1202 4 2.120465878850112 1202 5 1.790144450135621 1202 6 1.6038438933330426 1202 7 1.474042983920043 1202 8 1.4346634659107735 1202 9 1.6459331715102223 1202 10 1.66636614885752 1202 11 1.6336425448898524 1202 12 1.587727590998142 1202 13 1.6157493822559346 1202 14 1.8046457131661242 1202 15 1.7377726499613928 1202 16 1.7912640056920637 1202 17 1.735710391417804 1202 18 1.9136181113686845 1202 19 1.8730599184916983 1202 20 1.980742461367413 1202 21 2.0042908169273588 1202 22 1.9146376430123198 1202 23 1.8503012728379673 1202 24 2.0061015798958337 1202 25 1.9818301385026444 1203 1 4.136861709656426 1203 2 2.936756478811727 1203 3 1.9413516993597852 1203 4 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-0.3676554134274781 2314 10 -0.3616178163250723 2314 11 -0.27738443622914843 2314 12 -0.3260159583727926 2314 13 -0.3960628745232384 2314 14 -0.41266634507324085 2314 15 -0.5380385775506724 2314 16 -0.6161354598395548 2314 17 -0.3405022808973257 2314 18 -0.4761199271346328 2314 19 -0.48475418315685204 2314 20 -0.5510935972135087 2314 21 -0.43002390676151947 2314 22 -0.3583176363276337 2314 23 -0.36077161941408065 2314 24 -0.41869567784252837 2314 25 -0.4342893790130802 2315 1 1.0872116375216159 2315 2 0.8655421178499836 2315 3 0.4446450236110415 2315 4 0.29755757953989814 2315 5 0.1782703079991137 2315 6 0.10546301505567612 2315 7 0.1629441318178948 2315 8 -0.01071390660087701 2315 9 -1.2491202892313211 2315 10 -0.6692868765046427 2315 11 -0.40315732921731495 2315 12 -0.4892105903749666 2315 13 -0.18191373201804595 2315 14 -0.33689525008890797 2315 15 -0.12168286353274027 2315 16 -0.17099821858951714 2315 17 -0.3285846524261231 2315 18 -0.23070893023714945 2315 19 -0.294703647772522 2315 20 -0.13470039290289293 2315 21 -0.14797831397495997 2315 22 -0.1056713189837808 2315 23 -0.26117613202114853 2315 24 -0.16437882366064116 2315 25 0.026583991785038563 2316 1 3.3689496492686537 2316 2 1.6812269400675568 2316 3 0.3326802210030877 2316 4 -0.6572346303204917 2316 5 -0.5767312376635019 2316 6 -0.6992788905121472 2316 7 -0.667716069087021 2316 8 -0.7495962207856905 2316 9 -0.816532100591786 2316 10 -0.7062266518683415 2316 11 -0.6802113899384352 2316 12 -0.7608842463510612 2316 13 -0.8161250225370438 2316 14 -0.8651672617297734 2316 15 -0.9108663477373184 2316 16 -0.6800343183987323 2316 17 -0.7483593916150753 2316 18 -0.7117056484363147 2316 19 -0.7266723223434042 2316 20 -0.7440805353536533 2316 21 -0.8191019014110665 2316 22 -0.6916616827921729 2316 23 -0.8182933931694336 2316 24 -1.0505296241265256 2316 25 -1.2526961978162419 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104929.0 3 6.0 4 920.0 5 4087.0 6 4922.0 7 4478.0 8 4017.0 9 3849.0 10 3876.0 11 3894.0 12 3931.0 13 3979.0 14 3924.0 15 4057.0 16 4060.0 17 4368.0 18 4956.0 19 6086.0 20 7628.0 21 9121.0 22 10748.0 23 12790.0 24 14774.0 25 16851.0 26 19021.0 27 21468.0 28 24455.0 29 29361.0 30 36120.0 31 45733.0 32 59286.0 33 78138.0 34 107362.0 35 157497.0 36 258342.0 37 334018.0 38 691456.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.442945555993965 20.52436359082565 11.960854860046135 24.071835993134243 2 14.331798156454742 20.397254391535803 42.34455500976426 22.9263924422452 3 18.47771912545467 25.403067449325295 29.92645795240926 26.19275547281077 4 12.0446344724383 17.248308763018347 39.02769997006155 31.679356794481805 5 12.719994136979572 38.25116380003674 36.13714518494688 12.891696878036802 6 30.961704898928012 37.60310237829325 18.588800627635578 12.846392095143155 7 27.37206067266188 31.837537271181443 22.906965975457112 17.883436080699568 8 24.456885969988868 37.76284032597559 20.92529321179506 16.85498049224048 9 26.501872459417346 15.001819123849 20.767013093882028 37.72929532285163 10 15.264265197873367 28.834574599518476 35.01122876544521 20.889931437162954 11 33.668940281546114 22.174148394195022 25.76057868870488 18.396332635553993 12 23.668699912860877 24.615183158817004 32.34685180158945 19.369265126732664 13 29.296630930065987 22.338899556530883 25.928894451302213 22.435575062100916 14 20.4180724337567 23.106238843559314 28.04936361852545 28.42632510415853 15 22.39486627142231 32.28348933412128 23.531225575233048 21.790418819223365 16 21.3490314736443 28.098534579175038 29.07899977363962 21.47343417354105 17 21.15545796096609 29.15828327975766 28.440730628585676 21.245528130690573 18 21.003194367777606 27.130455655821756 31.353050747440147 20.51329922896049 19 23.37856704773587 26.851998232428087 28.051585291058906 21.717849428777136 20 23.58215919966859 27.4209691780985 28.795026974520628 20.20184464771228 21 22.52579769728225 26.75740115050157 27.731250529861317 22.98555062235486 22 23.0653680768094 28.69950325600908 27.766034533718525 20.469094133463 23 21.565908199200326 28.39654901990697 28.715388535602713 21.32215424528999 24 23.123347396154045 26.716420329005185 29.316024561958702 20.844207712882064 25 22.36528503461589 27.626013911197756 28.924163518792135 21.084537535394222 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2091.0 1 2091.0 2 1780.5 3 1470.0 4 1470.0 5 1470.0 6 2667.0 7 3864.0 8 3864.0 9 3864.0 10 4897.5 11 5931.0 12 5931.0 13 5931.0 14 8767.0 15 11603.0 16 11603.0 17 11603.0 18 20135.5 19 28668.0 20 28668.0 21 28668.0 22 44646.0 23 60624.0 24 60624.0 25 60624.0 26 88184.0 27 115744.0 28 115744.0 29 115744.0 30 147890.5 31 180037.0 32 180037.0 33 180037.0 34 199082.0 35 218127.0 36 218127.0 37 218127.0 38 228576.5 39 239026.0 40 239026.0 41 239026.0 42 248988.5 43 258951.0 44 258951.0 45 258951.0 46 263042.5 47 267134.0 48 267134.0 49 267134.0 50 253782.0 51 240430.0 52 240430.0 53 240430.0 54 210785.5 55 181141.0 56 181141.0 57 181141.0 58 154664.5 59 128188.0 60 128188.0 61 128188.0 62 105270.0 63 82352.0 64 82352.0 65 82352.0 66 63437.5 67 44523.0 68 44523.0 69 44523.0 70 32775.0 71 21027.0 72 21027.0 73 21027.0 74 14799.5 75 8572.0 76 8572.0 77 8572.0 78 5873.0 79 3174.0 80 3174.0 81 3174.0 82 2170.5 83 1167.0 84 1167.0 85 1167.0 86 799.0 87 431.0 88 431.0 89 431.0 90 293.0 91 155.0 92 155.0 93 155.0 94 105.0 95 55.0 96 55.0 97 55.0 98 39.0 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.381166524432314 2 0.18959300701161505 3 0.18080235380430962 4 0.16778268364862475 5 0.1521495760529302 6 0.18655191617233102 7 0.4621507734824481 8 0.24171920467871824 9 0.21838833589608592 10 0.1947723648472707 11 0.19173127400798665 12 0.20993030199932716 13 0.2233301085099225 14 0.22922222201103537 15 0.2959361522978292 16 0.4990239999087672 17 0.5029679145909638 18 0.23164559127358983 19 0.2115458815076968 20 0.20802962022477464 21 0.2329285514714128 22 0.32539671980339346 23 0.3279151231546756 24 0.29954744766947905 25 0.3355178502528857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2104508.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.36357273604035 #Duplication Level Percentage of deduplicated Percentage of total 1 85.99629867622227 62.229994142870616 2 9.796029468757414 14.17751381973644 3 2.2548822871084515 4.895140151831445 4 0.7516039943287139 2.1755500124921743 5 0.35901196050106693 1.2989694058413703 6 0.19928959303082525 0.8652784176493203 7 0.1276758124215606 0.6467354557160452 8 0.08944626618673146 0.5178121111336617 9 0.06433789041325742 0.4190147651342833 >10 0.31347717052822444 4.081722117865884 >50 0.022559780039345445 1.1266116682989142 >100 0.02121334731336532 3.1690935894318657 >500 0.002318751749326538 1.22436272441107 >1k 0.0017225012994996388 2.601848693340989 >5k 1.3250009996151066E-4 0.5703529242459785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6745 0.3205024642339207 No Hit GTCCTACAGTGGACATTTCTAAATT 5152 0.2448078125623661 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4933 0.23440157984669102 No Hit CTGTAGGACGTGGAATATGGCAAGA 4815 0.228794568611761 No Hit GGTATCAACGCAGAGTACTTTTTTT 4416 0.20983526791059953 No Hit CTTTAGGACGTGAAATATGGCGAGG 4375 0.2078870690916832 No Hit TATCAACGCAGAGTACTTTTTTTTT 4088 0.19424967735926876 No Hit GTCCTACAGTGTGCATTTCTCATTT 2372 0.11271042923096514 No Hit CTGAAGGACCTGGAATATGGCGAGA 2325 0.11047712814586591 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.75170443638133E-5 0.0 5 0.0 0.0 0.0 4.75170443638133E-5 0.0 6 0.0 0.0 0.0 4.75170443638133E-5 0.0 7 0.0 0.0 0.0 4.75170443638133E-5 0.0 8 0.0 0.0 0.0 4.75170443638133E-5 0.0 9 0.0 0.0 0.0 4.75170443638133E-5 4.75170443638133E-5 10 0.0 0.0 0.0 1.425511330914399E-4 4.75170443638133E-5 11 0.0 0.0 0.0 1.425511330914399E-4 4.75170443638133E-5 12 0.0 0.0 0.0 1.425511330914399E-4 4.75170443638133E-5 13 0.0 0.0 0.0 1.425511330914399E-4 4.75170443638133E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1280 0.0 14.273931 1 TATTCCG 40 0.005345114 14.2236595 5 TAGGACC 880 0.0 11.745294 4 GTTTAGG 290 0.0 11.578779 1 AATGGCG 60 0.005925527 11.071046 16 AACGGGG 120 1.2956298E-7 11.071046 16 GGACCGT 60 0.0059582926 11.062846 6 ACGTTAA 60 0.006107124 11.026228 8 CCACCTT 765 0.0 10.911815 13 TTAGGAC 1940 0.0 10.851298 3 TATAGCG 70 0.0015056427 10.845106 16 AGGACGT 3300 0.0 10.832968 5 AGGACCT 1655 0.0 10.714297 5 ACCTTTT 825 0.0 10.698241 15 CACCTTT 800 0.0 10.676672 14 TAGAAAT 835 0.0 10.674842 4 GGACGTG 3265 0.0 10.6586685 6 GTGGTAT 380 0.0 10.655703 1 GACGTGG 1610 0.0 10.54284 7 GACGTGA 1690 0.0 10.492654 7 >>END_MODULE