FastQCFastQC Report
Thu 26 May 2016
SRR1512405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104508
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83500.3967673204378411No Hit
GGTATCAACGCAGAGTACTTTTTTT59330.2819186242105043No Hit
CTGTAGGACGTGGAATATGGCAAGA56340.2677110279457241No Hit
TATCAACGCAGAGTACTTTTTTTTT55930.26576282912680776No Hit
GTCCTAAAGTGTGTATTTCTCATTT53100.2523155055718486No Hit
GTCCTACAGTGGACATTTCTAAATT53010.2518878521725743No Hit
CTTTAGGACGTGAAATATGGCGAGG52030.2472311818249206No Hit
CTGAAGGACCTGGAATATGGCGAGA27140.1289612584033893No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26260.12477975849937371No Hit
GTCCTACAGTGTGCATTTCTCATTT25950.12330673012409551No Hit
ATTTAGAAATGTCCACTGTAGGACG23230.11038209405713828No Hit
TTTCTAAATTTTCCACCTTTTTCAG22230.10563038962075696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.014.1689331
TCCAACG2000.013.29379618
TAGGACC8300.013.2873334
CCGTTTC1103.83352E-812.08699313
CCAACGA1406.85759E-1011.530333519
GATATAC4800.011.495391
CCAACGT1001.941322E-611.39468219
GGCGAGG14000.011.19120519
GCGCCAC855.3423057E-511.1728513
CGTTTCC1451.2259989E-911.13326514
AAATGTC7150.011.0262347
AGGACCT18450.010.8728655
GGACGTA700.001493744910.8554326
TAGAAAT8200.010.7826874
CCACCTT7900.010.57885113
TGTAGGA32100.010.3953552
GGACCTG18450.010.3479626
GTAGGAC31650.010.2730633
AATGTCC8150.010.2560528
CCGGTTT1303.8585495E-710.22769712
Produced by FastQC (version 0.11.5)