FastQCFastQC Report
Thu 26 May 2016
SRR1512404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570553
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80780.3142514470621691No Hit
CTGTAGGACGTGGAATATGGCAAGA75830.2949948902045591No Hit
GTCCTAAAGTGTGTATTTCTCATTT71060.2764385717781349No Hit
GTATCAACGCAGAGTACTTTTTTTT68930.2681524170091027No Hit
CTTTAGGACGTGAAATATGGCGAGG61320.23854789222396894No Hit
GTCCTACAGTGTGCATTTCTCATTT43930.17089707934440565No Hit
GGTATCAACGCAGAGTACTTTTTTT41620.16191068614418766No Hit
TATCAACGCAGAGTACTTTTTTTTT39460.15350782496995782No Hit
CTGAAGGACCTGGAATATGGCGAGA32480.1263541346939744No Hit
CTGTAGGACCTGGAATATGGCGAGA30170.11736774149375641No Hit
GTCCTTCAGTGTGCATTTCTCATTT28770.11192144258453338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13350.013.5425311
TAGGACC13750.013.4504454
GTGTAGG4400.012.7982971
AGGACCT25450.011.1799085
AAATGTC10500.011.1103847
TAGAAAT11500.010.9687864
GTCCTAC39200.010.9630961
GTCCTAG4650.010.8354561
GGCGAGG18150.010.8062219
AATGTCC11050.010.7788128
GGACCGA1159.010855E-710.72158056
TGTAGGA45200.010.701092
GGACCTG25650.010.6862126
CTAGACG803.830602E-410.6698244
GTGTAGC4600.010.5236951
GTCCTAA22300.010.4996181
TCCAACG1909.094947E-1210.46809418
ATTTAGA10300.010.45488551
TCCTACA45000.010.4114372
GTATCAA29100.010.3546841
Produced by FastQC (version 0.11.5)