FastQCFastQC Report
Thu 26 May 2016
SRR1512404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2570553
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA91560.3561879486631865No Hit
GTATCAACGCAGAGTACTTTTTTTT88850.3456454700603333No Hit
GTCCTACAGTGGACATTTCTAAATT88520.3443616996031593No Hit
GTCCTAAAGTGTGTATTTCTCATTT78220.30429250048530415No Hit
CTTTAGGACGTGAAATATGGCGAGG71740.2790839169626147No Hit
GGTATCAACGCAGAGTACTTTTTTT57280.22283142965735386No Hit
TATCAACGCAGAGTACTTTTTTTTT56830.22108083357938935No Hit
GTCCTACAGTGTGCATTTCTCATTT47830.1860689120200984No Hit
CTGAAGGACCTGGAATATGGCGAGA39080.15202954383745443No Hit
CTGTAGGACCTGGAATATGGCGAGA35530.13821928588906746No Hit
GTCCTTCAGTGTGCATTTCTCATTT30880.1201297930834338No Hit
ATTTAGAAATGTCCACTGTAGGACG28640.11141571482867693No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27410.10663075221557385No Hit
GAATATGGCAAGAAAACTGAAAATC27330.10631953513504681No Hit
TTTCTAAATTTTCCACCTTTTTCAG26790.10421881984148935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG759.597825E-713.9438465
GGTATCA16400.013.4609621
ATACCGA604.097854E-412.6651236
TAGGACC12850.012.2816334
GGCGAGG20450.012.16422119
TCCAACG1800.011.60450218
GCCCTAT1104.8686707E-711.2456011
TAACGCT600.00584452611.0916964
ATATCGA600.005882763311.0819836
CGATCCA600.005882763311.08198310
TGTAGGA44950.010.9777722
TTAGGAC27850.010.9579933
TTGCGAG700.001499080310.85096318
AGGACGT47800.010.8397625
GGACGTG47250.010.7150966
CTGTAGG44950.010.69039251
ATTGCGA803.7923807E-410.681416517
TAGGACT4300.010.6126524
CTAGGAC3950.010.5902633
TAGGACG50800.010.5552024
Produced by FastQC (version 0.11.5)