FastQCFastQC Report
Thu 26 May 2016
SRR1512403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700048
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT91040.33717919088845827No Hit
CTGTAGGACGTGGAATATGGCAAGA82520.30562419631058413No Hit
GTCCTAAAGTGTGTATTTCTCATTT75540.2797728040390393No Hit
GTATCAACGCAGAGTACTTTTTTTT74870.27729136667199994No Hit
CTTTAGGACGTGAAATATGGCGAGG63650.2357365498687431No Hit
GTCCTACAGTGTGCATTTCTCATTT51250.18981144038920789No Hit
GGTATCAACGCAGAGTACTTTTTTT44340.1642193027679508No Hit
TATCAACGCAGAGTACTTTTTTTTT41890.15514539000788136No Hit
CTGTAGGACCTGGAATATGGCGAGA36260.13429390884902787No Hit
CTGAAGGACCTGGAATATGGCGAGA32790.12144228547048054No Hit
GTCCTTCAGTGTGCATTTCTCATTT29880.11066469929423477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15950.013.0206394
TCCAACG2200.012.91654118
GGTATCA14150.012.7068851
GTCTACG600.004390101411.5257971
GGACCTG28700.011.3671016
GTATTAG2000.011.3611421
TGTAGGA49550.011.2920672
CCAACGA2100.011.28012319
AGGACCT29900.011.2278645
GGCGTGC1105.3111035E-711.1702278
TACACCG855.4215558E-511.1569675
GTATTAA2750.011.1366141
GTCCTAC45750.011.0777221
GTAGGAC49000.010.954323
CTACACT5200.010.7596364
TATCGCG1502.1955202E-910.7484797
GTCCTAA21800.010.7402911
GTATCAA32450.010.6555911
GTGTAGG5750.010.6524131
ATCCTAC4000.010.6201981
Produced by FastQC (version 0.11.5)