FastQCFastQC Report
Thu 26 May 2016
SRR1512403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2700048
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA97040.3594010180559753No Hit
GTCCTACAGTGGACATTTCTAAATT95400.35332705196352066No Hit
GTATCAACGCAGAGTACTTTTTTTT90590.3355125538508945No Hit
GTCCTAAAGTGTGTATTTCTCATTT81950.30351312272967No Hit
CTTTAGGACGTGAAATATGGCGAGG75930.2812172228049279No Hit
GGTATCAACGCAGAGTACTTTTTTT60620.224514527149147No Hit
TATCAACGCAGAGTACTTTTTTTTT57530.2130702861578757No Hit
GTCCTACAGTGTGCATTTCTCATTT54390.2014408632735418No Hit
CTGTAGGACCTGGAATATGGCGAGA43160.15984901009167243No Hit
CTGAAGGACCTGGAATATGGCGAGA39800.1474047868778629No Hit
GTCCTTCAGTGTGCATTTCTCATTT30520.11303502752543658No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30250.11203504530289832No Hit
ATTTAGAAATGTCCACTGTAGGACG30020.11118320859481017No Hit
GAATATGGCAAGAAAACTGAAAATC29460.10910917139250857No Hit
GTACTGGTTCACTATCGGTCAGTCA27840.10310927805727897No Hit
TTTCTAAATTTTCCACCTTTTTCAG27020.10007229501105167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.014.0637951
TAGGACC16650.013.3037274
GGACCGA500.001501458313.298346
TCCAACG1352.7284841E-1112.66062918
GGCGAGG19300.011.906239519
CTAGGAC6000.011.7249793
TATACTG3550.011.5152065
CCAACGA1251.84682E-811.39456619
CGCCCTA2850.011.32751216
AGGACCT31150.011.2921145
GACGTGG24500.011.2432867
CGTAGGA855.2876574E-511.1844012
TAGAAAT11850.011.1513664
GACGTTA600.005883833411.0817457
ACCGTCC600.005883833411.0817458
CCCCGTA1803.6379788E-1211.07825615
AAATGTC11450.010.9503967
GGACCTG31100.010.9037846
CGGCAAA700.001497964610.85196817
GTAAGAC2200.010.8031133
Produced by FastQC (version 0.11.5)