FastQCFastQC Report
Thu 26 May 2016
SRR1512402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941184
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65860.3392774718934424No Hit
GTATCAACGCAGAGTACTTTTTTTT65240.3360835448880683No Hit
GTCCTAAAGTGTGTATTTCTCATTT61440.3165078632422274No Hit
CTGTAGGACGTGGAATATGGCAAGA56900.29312007517061756No Hit
CTTTAGGACGTGAAATATGGCGAGG51140.2634474629916587No Hit
GGTATCAACGCAGAGTACTTTTTTT42320.218011275592628No Hit
GTCCTACAGTGTGCATTTCTCATTT38850.20013558735287332No Hit
TATCAACGCAGAGTACTTTTTTTTT37780.1946234875210181No Hit
CTGAAGGACCTGGAATATGGCGAGA27080.13950248920246613No Hit
CTGTAGGACCTGGAATATGGCGAGA25470.13120858197883356No Hit
GTCCTTCAGTGTGCATTTCTCATTT24840.1279631400217599No Hit
ATTTAGAAATGTCCACTGTAGGACG22710.11699045530974911No Hit
GAATATGGCAAGAAAACTGAAAATC21820.11240562460848637No Hit
TTTCTAAATTTTCCACCTTTTTCAG21510.11080866110579933No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19640.10117536513797765No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG602.5992284E-514.23226816
CTAGGCG400.0053372514.2267374
GTCCTAG3200.013.8938221
ATACGGC903.6992787E-813.70550215
GGTATCA12650.013.2775391
ACCGTGC500.00155731813.23370558
CTAGTAC1102.8157956E-912.9333963
TAGGACC11500.012.7834434
GGCGAGG16550.011.90897619
GTATAAG2050.011.566891
TCCTAGA3650.011.4330632
GTGGTAT5200.011.4000591
TGGTATC5050.011.2684112
GTGTAGG3600.011.252281
AATACGG1105.048896E-711.213593514
ACCTTTT9500.011.08647515
GACCTCG600.005948423411.065247
CACCTTT9800.010.94073514
TGTAGGA35700.010.8657042
CCGTGCA700.00154948410.807779
Produced by FastQC (version 0.11.5)