FastQCFastQC Report
Thu 26 May 2016
SRR1512402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941184
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83880.43210741485608783No Hit
GTCCTACAGTGGACATTTCTAAATT68420.35246529952853517No Hit
CTGTAGGACGTGGAATATGGCAAGA67490.34767440902047414No Hit
GTCCTAAAGTGTGTATTTCTCATTT63670.327995697471234No Hit
CTTTAGGACGTGAAATATGGCGAGG62640.32268965744617717No Hit
GGTATCAACGCAGAGTACTTTTTTT56940.29332613497741583No Hit
TATCAACGCAGAGTACTTTTTTTTT52470.270298951567703No Hit
GTCCTACAGTGTGCATTTCTCATTT40260.20739919554251424No Hit
CTGAAGGACCTGGAATATGGCGAGA32830.16912358642972536No Hit
CTGTAGGACCTGGAATATGGCGAGA30950.15943877551020408No Hit
ATTTAGAAATGTCCACTGTAGGACG26310.13553583792159835No Hit
GTCCTTCAGTGTGCATTTCTCATTT25970.1337843295638126No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25930.13357826975701426No Hit
TTTCTAAATTTTCCACCTTTTTCAG25150.12956010352444694No Hit
GAATATGGCAAGAAAACTGAAAATC24680.12713890079456663No Hit
GGAATATGGCGAGAAAACTGAAAAT20970.10802685371402196No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19560.100763245524381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAG400.00529780914.24265918
GGTATCA14650.013.183191
TAGGACC11950.013.1262884
GGCGAGG16450.011.65964419
TGGCGAA1406.85759E-1011.52977218
TGCGCAT600.005882191511.08190510
GAACCGA600.00589913711.07762219
CCGTACA1553.1650416E-1011.02657417
AGGACCT22600.010.9370975
GGACCTG22100.010.7887596
CTGCGCA803.7742787E-410.6866749
GTCCCGC3200.010.68502112
CCCCGTA1605.493348E-1010.68226915
TGTCCCG3050.010.58852711
CGCCCTA3150.010.550117516
TGGTATC4450.010.4679582
TGTAGGA38950.010.3974792
TCCCGCC3200.010.38607413
GGCGAAA1106.083854E-610.35829619
AATGTCC8750.010.3134898
Produced by FastQC (version 0.11.5)