FastQCFastQC Report
Thu 26 May 2016
SRR1512401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954905
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73300.374954281665861No Hit
GTCCTACAGTGGACATTTCTAAATT69190.3539302421345283No Hit
GTCCTAAAGTGTGTATTTCTCATTT68630.35106565280665813No Hit
CTGTAGGACGTGGAATATGGCAAGA65360.33433849726713066No Hit
CTTTAGGACGTGAAATATGGCGAGG58280.2981219036219151No Hit
GGTATCAACGCAGAGTACTTTTTTT45610.23331057007885295No Hit
TATCAACGCAGAGTACTTTTTTTTT42840.21914108358206666No Hit
GTCCTACAGTGTGCATTTCTCATTT40410.20671081203434438No Hit
CTGAAGGACCTGGAATATGGCGAGA28530.14594059557881328No Hit
CTGTAGGACCTGGAATATGGCGAGA25840.1321803361288656No Hit
GTCCTTCAGTGTGCATTTCTCATTT25550.1306968880840757No Hit
ATTTAGAAATGTCCACTGTAGGACG24950.12762768523278623No Hit
GAATATGGCAAGAAAACTGAAAATC22990.1176016225852407No Hit
TTTCTAAATTTTCCACCTTTTTCAG21130.10808709374624344No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20130.10297175566076101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCGT250.006091460618.9680886
GGTATCA12550.013.8532951
TATAGCG551.9830653E-413.7995916
TAGGACC11400.013.3109394
GACCGTG500.001518899513.2776627
TAGAAAT9700.012.4172544
AATGTCC9000.012.2887768
GAAATGT9500.011.8800136
GACGGGG802.916968E-511.8550557
GGCGAGG18000.011.84530119
AAATGTC9300.011.829567
GGACGGG1407.003109E-1011.5163396
CTACACT2850.011.3142984
AGGACCT21950.011.2774455
GTCCTAT2900.011.2408741
TTTAGAA10650.011.220562
ATTTAGA8750.011.1766411
GTCCTAA19700.011.0817961
ATGTCCA10000.011.0647239
GGACCTG22200.011.02199656
Produced by FastQC (version 0.11.5)