FastQCFastQC Report
Thu 26 May 2016
SRR1512401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1954905
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88810.4542931753716933No Hit
CTGTAGGACGTGGAATATGGCAAGA78200.4000194382847248No Hit
GTCCTACAGTGGACATTTCTAAATT74930.38329228274519733No Hit
GTCCTAAAGTGTGTATTTCTCATTT71230.3643655318289124No Hit
CTTTAGGACGTGAAATATGGCGAGG68530.3505541189981099No Hit
GGTATCAACGCAGAGTACTTTTTTT60220.30804565950775104No Hit
TATCAACGCAGAGTACTTTTTTTTT55240.2825712758420486No Hit
GTCCTACAGTGTGCATTTCTCATTT44680.2285533056593543No Hit
CTGAAGGACCTGGAATATGGCGAGA34760.17780915185136872No Hit
CTGTAGGACCTGGAATATGGCGAGA30660.15683626570089085No Hit
ATTTAGAAATGTCCACTGTAGGACG29510.15095362690258604No Hit
GTCCTTCAGTGTGCATTTCTCATTT28460.14558252191282953No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27240.13934180944854097No Hit
TTTCTAAATTTTCCACCTTTTTCAG27020.13821643506973486No Hit
GAATATGGCAAGAAAACTGAAAATC26300.1345333916481875No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21870.11187244392950042No Hit
GTACTGGTTCACTATCGGTCAGTCA20360.10414828342042197No Hit
GGAATATGGCGAGAAAACTGAAAAT20140.10302290904161585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15400.014.2756511
GCGCCTT1051.380613E-913.565613
TAGGACC13150.013.5203054
CCGTTCA500.001502043113.2973499
CTAGGAC2600.013.1643613
ATCGCGC1102.755769E-912.9519628
CGCGCCT1102.757588E-912.951312
GGACCGT604.0988668E-412.6644666
TCGCGCG1155.342372E-912.3888339
ATACCGT550.00307292812.0888086
GCAATAC657.971427E-411.7016543
ACCGTGC752.0781801E-411.3977288
GGCGAGG17050.011.30483619
GTCGAGG1451.2278178E-911.13200919
GTCGAAA600.005899888511.07743915
ATATCGC600.005899888511.07743915
ACCTTTT9300.011.02639115
TATCGCG1303.2767275E-810.95935257
TAGAAAT10250.010.9453294
GGACGTA700.001493863310.8552576
Produced by FastQC (version 0.11.5)