FastQCFastQC Report
Thu 26 May 2016
SRR1512400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944573
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT60820.31276789300273117No Hit
GTATCAACGCAGAGTACTTTTTTTT58970.30325423627706444No Hit
GTCCTAAAGTGTGTATTTCTCATTT58590.301300079760441No Hit
CTGTAGGACGTGGAATATGGCAAGA58260.299603049101268No Hit
CTTTAGGACGTGAAATATGGCGAGG48210.24792075175372694No Hit
GGTATCAACGCAGAGTACTTTTTTT35260.18132515467405955No Hit
TATCAACGCAGAGTACTTTTTTTTT34210.17592551166760004No Hit
GTCCTACAGTGTGCATTTCTCATTT30410.15638394650136558No Hit
CTGAAGGACCTGGAATATGGCGAGA25780.13257409210145363No Hit
GTCCTTCAGTGTGCATTTCTCATTT23710.12192908160300486No Hit
ATTTAGAAATGTCCACTGTAGGACG22430.11534665965227328No Hit
CTGTAGGACCTGGAATATGGCGAGA20360.10470164915382452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11500.014.0031331
TACACCG500.001519449613.2770195
TAGGACC8450.013.2426634
GTGCGCC905.556558E-712.6382411
GGCCCGT550.003108914712.0700176
CAACGGA550.003110119612.0693924
CCACCTT7100.012.02393313
CACCTTT7200.011.99271314
GTCCTAG2650.011.5571641
GTCATAG1251.3049976E-711.0650531
AACGGAG600.005952662311.0641815
TATGTCG951.3805373E-510.98553916
GTATTGG1801.6370905E-1110.9772351
AGGACCT19100.010.9731535
GGCGAGG14550.010.94247419
CTAGGAC2800.010.8381013
TTAGGAC21400.010.8127783
ACCTTTT7900.010.80995715
GTACCGC650.00796194310.6394741
GTCCTAC30500.010.5921311
Produced by FastQC (version 0.11.5)