FastQCFastQC Report
Thu 26 May 2016
SRR1512400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1944573
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT76040.3910370040106491No Hit
CTGTAGGACGTGGAATATGGCAAGA67740.3483541116738739No Hit
GTCCTACAGTGGACATTTCTAAATT64970.3341093391711188No Hit
GTCCTAAAGTGTGTATTTCTCATTT62150.31960744081091325No Hit
CTTTAGGACGTGAAATATGGCGAGG57670.29656896398335264No Hit
TATCAACGCAGAGTACTTTTTTTTT48400.24889783001203863No Hit
GGTATCAACGCAGAGTACTTTTTTT46950.2414411801459755No Hit
GTCCTACAGTGTGCATTTCTCATTT32660.16795461008663598No Hit
CTGAAGGACCTGGAATATGGCGAGA31680.1629149432806071No Hit
ATTTAGAAATGTCCACTGTAGGACG25710.132214115901023No Hit
GTCCTTCAGTGTGCATTTCTCATTT23710.12192908160300486No Hit
CTGTAGGACCTGGAATATGGCGAGA23440.12054060197277243No Hit
TTTCTAAATTTTCCACCTTTTTCAG22880.11766079236932735No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22540.11591233653866428No Hit
GAATATGGCAAGAAAACTGAAAATC22300.11467813242290209No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG400.005302414.24080118
GGTATCA12500.014.01244451
TAGGACC7250.012.9834034
GTCGAGA604.1152E-412.6584919
CGCGCCT1353.7289283E-1011.95923812
GTATCGC1750.011.9412886
GGCGAGG16750.011.27928219
CCACCTT7700.011.22348913
TGTATCG1950.011.214625
GGATCGT600.005882302811.0818786
GATCGTG600.00588342911.08159357
CTAATAC1553.092282E-1011.0416033
TATCGCG1553.1468517E-1011.0305247
TATGTCG1553.1650416E-1011.02513616
GTATCAA28550.010.9363851
GCGCGCC1502.1409505E-910.76442211
CACCTTT8350.010.68853214
ATCGCGC1605.456968E-1010.6858228
CCGCCCT2400.010.6811515
AGGACCT18650.010.6041495
Produced by FastQC (version 0.11.5)