FastQCFastQC Report
Thu 26 May 2016
SRR1512399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309356
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61750.26739056256376237No Hit
GTCCTAAAGTGTGTATTTCTCATTT40220.17416110811845378No Hit
GGTATCAACGCAGAGTACTTTTTTT39910.17281874254121063No Hit
TATCAACGCAGAGTACTTTTTTTTT37920.1642016215776173No Hit
GTCCTACAGTGGACATTTCTAAATT37860.16394180888524768No Hit
CTTTAGGACGTGAAATATGGCGAGG34500.1493922981125474No Hit
CTGTAGGACGTGGAATATGGCAAGA33880.14670756695806103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14400.013.3100561
TAGGACC7550.012.0597774
GGCGAGG10150.011.76260419
GTCCTAT2950.011.7215941
TTAGGAC13950.010.9462833
TCTACGC700.001512313410.8394433
AGGACGT23000.010.7216235
GGACGTG22400.010.7124196
GCCCTAT1552.2148015E-810.1983261
GACGTGG12200.010.1844117
TTTAGGA16100.010.1325232
GACGTGA11650.010.0953247
GTATCAA29600.010.0798911
TGGCGAG23450.010.01874418
ATAGGAC2950.09.9667763
AAATGTC7150.09.9490067
TTTAGAA9250.09.9459212
TAGGACG25500.09.9308424
ATTTAGA7900.09.8796281
GCGCCAC1252.6244306E-69.86410813
Produced by FastQC (version 0.11.5)