FastQCFastQC Report
Thu 26 May 2016
SRR1512399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309356
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78240.3387957508500205No Hit
GGTATCAACGCAGAGTACTTTTTTT55350.23967720871099996No Hit
TATCAACGCAGAGTACTTTTTTTTT54050.2340479337096576No Hit
GTCCTAAAGTGTGTATTTCTCATTT42850.18554956446732335No Hit
CTGTAGGACGTGGAATATGGCAAGA39150.1695277817711951No Hit
CTTTAGGACGTGAAATATGGCGAGG39060.16913806273264062No Hit
GTCCTACAGTGGACATTTCTAAATT38390.16623682100117956No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24740.10712943348708472No Hit
GTCCTACAGTGTGCATTTCTCATTT23860.10331884733232988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15500.013.630931
TAGGACC8750.012.2767884
GGCGAGG12750.011.3940519
ACCGTCC700.001494063810.8552378
TGGCGAG23650.010.84000618
TTAGGAC17350.010.3008423
AGGACGT24750.010.2939825
TATACTG3050.010.2857955
GATATAC4300.010.1810731
ATTTAGA8250.010.1515641
CCGGTTT750.00265369410.13089612
GGACGTG24800.010.1113476
AAGTCGT2350.010.1046097
AGGACCT15900.010.10446555
TAGAAAT8400.010.0722174
AATGTCC7650.010.0570588
TATGTCG951.6532937E-49.994781516
TGTCGAG951.6532937E-49.994781518
GACGTGA14550.09.9879377
TGTCCAC9200.09.80806110
Produced by FastQC (version 0.11.5)