FastQCFastQC Report
Thu 26 May 2016
SRR1512398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82180.3520695330901663No Hit
GTCCTACAGTGGACATTTCTAAATT64590.2767117442479173No Hit
CTGTAGGACGTGGAATATGGCAAGA59840.25636214237181254No Hit
GTCCTAAAGTGTGTATTTCTCATTT54740.23451309614694213No Hit
GGTATCAACGCAGAGTACTTTTTTT52110.2232458428976462No Hit
CTTTAGGACGTGAAATATGGCGAGG49620.21257836738785654No Hit
TATCAACGCAGAGTACTTTTTTTTT47200.20221078074782No Hit
GTCCTACAGTGTGCATTTCTCATTT41960.1797619567834434No Hit
CTGTAGGACCTGGAATATGGCGAGA27110.11614267512867374No Hit
CTGAAGGACCTGGAATATGGCGAGA24950.10688896143343453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11950.013.4127754
GGTATCA16750.013.3869351
AGGACCT24200.011.326155
GGCGAGG16300.011.15952619
GGACCTG23200.011.0376246
ACCGTCC600.00610390811.0270918
GTCCTAG3000.010.8658281
AAATGTC9250.010.7658277
CTAGGAC3000.010.7487393
TGTAGGA38050.010.7179982
CCGGTAC803.830715E-410.66970353
GTAGGAC37300.010.5521063
TAGAAAT9800.010.5487324
AGGACGT37300.010.5266795
AATGTCC9800.010.5127098
GTCCTAA18650.010.4871251
GGACGTG37650.010.4792016
GTCCTAC32350.010.4734541
GTATCAA35450.010.3099271
ATGTCCA10600.010.2595919
Produced by FastQC (version 0.11.5)