FastQCFastQC Report
Thu 26 May 2016
SRR1512398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334198
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT100450.43034052809573137No Hit
CTGTAGGACGTGGAATATGGCAAGA68650.29410529869359836No Hit
GTCCTACAGTGGACATTTCTAAATT68250.29239164800929485No Hit
GGTATCAACGCAGAGTACTTTTTTT66820.2862653468129096No Hit
TATCAACGCAGAGTACTTTTTTTTT65180.27923937900726503No Hit
GTCCTAAAGTGTGTATTTCTCATTT59680.2556766820980911No Hit
CTTTAGGACGTGAAATATGGCGAGG58740.25164960298997774No Hit
GTCCTACAGTGTGCATTTCTCATTT44670.19137194016960002No Hit
CTGTAGGACCTGGAATATGGCGAGA31780.1361495468679178No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31240.133836118444108No Hit
CTGAAGGACCTGGAATATGGCGAGA29630.12693867443978615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.013.3716341
TAGGACC12550.013.2572724
CATACCG550.003051492412.1002735
AGGACCT23750.011.4888925
GTGGTAT5500.011.24706651
GTCCTAT3300.011.2470661
TCCGAAC600.005843383711.0919173
GGACCTG23250.011.0714836
GGCGAGG17400.010.91324919
GTGCCGT700.00149426210.85507311
TGTAGGA37650.010.8330842
TGGTATC5300.010.7630462
AAATGTC8150.010.7223427
AATGTCC8350.010.693038
GTATCAA35250.010.5021711
CTGTAGG37500.010.4811121
GTAGGAC38100.010.2809223
CTAATAC2250.010.1411823
TTAGGAC23400.010.1168043
AGGACGT37350.010.1055455
Produced by FastQC (version 0.11.5)