FastQCFastQC Report
Thu 26 May 2016
SRR1512397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824804
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58900.32277439111268935No Hit
GTCCTACAGTGGACATTTCTAAATT57060.31269111641579045No Hit
GTCCTAAAGTGTGTATTTCTCATTT55560.3044710555215793No Hit
CTGTAGGACGTGGAATATGGCAAGA53290.2920313633683398No Hit
CTTTAGGACGTGAAATATGGCGAGG47030.2577263092364988No Hit
GGTATCAACGCAGAGTACTTTTTTT39590.21695480720121174No Hit
TATCAACGCAGAGTACTTTTTTTTT36390.19941867729356139No Hit
GTCCTACAGTGTGCATTTCTCATTT32890.1802385352070688No Hit
CTGAAGGACCTGGAATATGGCGAGA24620.13491859947698492No Hit
CTGTAGGACCTGGAATATGGCGAGA22940.12571213127546849No Hit
GTCCTTCAGTGTGCATTTCTCATTT22790.12489012518604739No Hit
ATTTAGAAATGTCCACTGTAGGACG22420.12286251016547531No Hit
GAATATGGCAAGAAAACTGAAAATC19710.10801160014993391No Hit
TTTCTAAATTTTCCACCTTTTTCAG19630.10757319690224265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG400.005363345614.21621510
CGGTAGG450.00889153113.1708231
TAGGACC9900.013.1239694
GGTATCA9950.012.7075281
GGCGAGG14250.011.97089119
GATCTAG700.001076542311.2892771
AGGACCT20450.011.1300675
GTCCTAC27250.010.9837421
GGACCTG20050.010.9264096
TAGAAAT9350.010.8530554
GTATCAA21600.010.7012931
TCCTACA29900.010.5621622
AATGTCC9050.010.5503478
GCGCAGA854.6031288E-410.4591841
GTATAGG1707.912604E-1010.4591841
AGGACCG1002.4447449E-510.4321185
TGGATAG1002.4447449E-510.4321185
GTCGAGG1002.4641391E-510.4246519
TGTAGGA32900.010.3773492
ACGAAAT1856.184564E-1110.24874212
Produced by FastQC (version 0.11.5)