FastQCFastQC Report
Thu 26 May 2016
SRR1512397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824804
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74900.4104550406509411No Hit
CTGTAGGACGTGGAATATGGCAAGA62740.3438177470018698No Hit
GTCCTACAGTGGACATTTCTAAATT61020.33439207717650776No Hit
GTCCTAAAGTGTGTATTTCTCATTT58580.32102077812192437No Hit
CTTTAGGACGTGAAATATGGCGAGG54700.2997582206088983No Hit
GGTATCAACGCAGAGTACTTTTTTT53520.2932917727054522No Hit
TATCAACGCAGAGTACTTTTTTTTT50660.2776188566004897No Hit
GTCCTACAGTGTGCATTTCTCATTT35570.194925044004726No Hit
CTGAAGGACCTGGAATATGGCGAGA29420.16122279433846046No Hit
CTGTAGGACCTGGAATATGGCGAGA27300.1496051082746421No Hit
ATTTAGAAATGTCCACTGTAGGACG25810.1414398477863924No Hit
TTTCTAAATTTTCCACCTTTTTCAG23660.1296577605046898No Hit
GTCCTTCAGTGTGCATTTCTCATTT23620.1294385588808442No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22840.1251641272158544No Hit
GAATATGGCAAGAAAACTGAAAATC21110.11568365698453095No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19080.10455917457436524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAA250.005979275319.0291581
TAGGACC8400.013.5814414
GGTATCA12850.013.3278141
TGTCGAG1352.7284841E-1112.66142518
GCGCCAC701.09448585E-412.21023413
GTCGAGG1103.837158E-812.08590519
GGCGAGG14550.011.87824319
AAATGTC8450.011.8017877
CTCGAAC1459.822543E-1111.78822218
AATGTCC8400.011.6459038
TGCGCCA907.4877644E-611.60757712
GCGTGCG1157.0998794E-811.5623329
AGGACGT35150.011.4949625
GGACGTG34750.011.4791496
TAGAAAT9500.011.4084114
ATAGGAC2900.011.1461493
GACGTGG17500.011.1257957
AGAACCG600.00584414711.0915115
TAAGGCG600.005893253711.0790516
TGTAGGA33600.011.0355262
Produced by FastQC (version 0.11.5)