FastQCFastQC Report
Thu 26 May 2016
SRR1512396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292884
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78460.34218913822068625No Hit
GTCCTACAGTGGACATTTCTAAATT51900.22635248883066042No Hit
CTGTAGGACGTGGAATATGGCAAGA47610.2076424276151781No Hit
GGTATCAACGCAGAGTACTTTTTTT46650.20345556076975546No Hit
GTCCTAAAGTGTGTATTTCTCATTT45750.19953037310217175No Hit
TATCAACGCAGAGTACTTTTTTTTT44420.1937298179934092No Hit
CTTTAGGACGTGAAATATGGCGAGG36890.1608890811746255No Hit
GTCCTACAGTGTGCATTTCTCATTT30190.13166823964928012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14100.014.1502761
TAGGACC9500.013.2777964
TAACCGT604.151424E-412.645526
AATCCCG1001.9954987E-611.36874819
GGCGAGG11950.011.25775619
TGTAGGA29700.011.1765972
ATACGGC600.00592928311.07017215
GTAGGAC28950.011.040263
CTAATAC2250.010.9594513
GGACCTG16300.010.9387646
AGGACCT18350.010.905475
AGGACGT28850.010.8812845
GTGTAGG5000.010.864891
GGACGTG29000.010.7595946
GTCCTAC27800.010.7298781
TGGCGAG26600.010.68257318
GTATTAT2500.010.6673471
AAATGTC7250.010.5963067
TTAGGAC15850.010.5911223
TAGGACA7300.010.5234984
Produced by FastQC (version 0.11.5)