FastQCFastQC Report
Thu 26 May 2016
SRR1512396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292884
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT97430.42492337161408944No Hit
GGTATCAACGCAGAGTACTTTTTTT65360.28505585105919007No Hit
TATCAACGCAGAGTACTTTTTTTTT62760.27371642001950386No Hit
CTGTAGGACGTGGAATATGGCAAGA58000.2529565385776167No Hit
GTCCTACAGTGGACATTTCTAAATT55630.24262021105297954No Hit
GTCCTAAAGTGTGTATTTCTCATTT49580.21623422728755576No Hit
CTTTAGGACGTGAAATATGGCGAGG43800.19102579982240708No Hit
GTCCTACAGTGTGCATTTCTCATTT33200.1447958117375323No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30850.13454671060550819No Hit
CTGAAGGACCTGGAATATGGCGAGA25240.11007970747756973No Hit
GTACTGGTTCACTATCGGTCAGTCA23650.10314520926483853No Hit
CTGTAGGACCTGGAATATGGCGAGA23380.1019676529645634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACC350.002160336616.2972984
GGTATCA18150.013.5252561
TAGGACC9650.013.2996094
CCGTCTT500.001501757313.2978489
GGACCTG18000.011.5036946
AGGACCT19150.011.3686435
TGTAGGA34500.011.2705762
CTGTAGG34300.010.9296061
GTACTAG1053.4036093E-610.8741261
GTGGTAT5550.010.8006521
GTAGGAC34900.010.7870383
GTCCTAA14150.010.3553951
AGGACGT33050.010.2980145
TATGTCG1201.5344376E-610.2864116
GGCGAGG12600.010.24873119
TGGCGAG28750.010.20521918
TAGGACT4100.010.2023744
GGACGTG32700.010.1375026
GTCCTAC27900.010.1287211
CGCCCTA4050.010.08126816
Produced by FastQC (version 0.11.5)