FastQCFastQC Report
Thu 26 May 2016
SRR1512395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428946
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61960.255090067873061No Hit
GTATCAACGCAGAGTACTTTTTTTT60860.2505613545957794No Hit
CTGTAGGACGTGGAATATGGCAAGA57970.2386631897127396No Hit
GTCCTAAAGTGTGTATTTCTCATTT56620.23310522341789403No Hit
CTTTAGGACGTGAAATATGGCGAGG46390.19098818993917527No Hit
GGTATCAACGCAGAGTACTTTTTTT37600.15479965384162514No Hit
TATCAACGCAGAGTACTTTTTTTTT35530.14627743885619524No Hit
GTCCTACAGTGTGCATTTCTCATTT34350.14141936461329316No Hit
CTGAAGGACCTGGAATATGGCGAGA24540.10103147620408193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10650.014.070464
TCCAACG2200.012.4879418
GGTATCA12300.012.3682661
TAGAACG701.1084691E-412.19397454
TAAACCG550.003107303512.0710535
TCGCGCG1104.0674422E-812.0359139
TGTAGGA36400.011.5428262
ATCGCGC1157.580638E-811.5083368
CCAACGA1657.2759576E-1211.48625119
GGCGAGG14400.011.3845519
CCGTTTC1251.879016E-811.381042513
GTAGGAC36850.011.0930153
AGGACGT35650.010.9343275
GGACGTG35500.010.8736636
GTCCTAG4100.010.8423111
TCGTAGA700.001512514810.8393132
GTCCTAC31450.010.8051561
CTGTAGG34050.010.7924251
AGGACCT21650.010.776735
GGACCTG21150.010.7624376
Produced by FastQC (version 0.11.5)