FastQCFastQC Report
Thu 26 May 2016
SRR1512395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2428946
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA69340.2854736169515502No Hit
GTATCAACGCAGAGTACTTTTTTTT67850.2793392689668688No Hit
GTCCTACAGTGGACATTTCTAAATT67000.27583980870715114No Hit
GTCCTAAAGTGTGTATTTCTCATTT60080.24735008518097973No Hit
CTTTAGGACGTGAAATATGGCGAGG57600.2371398952467449No Hit
GGTATCAACGCAGAGTACTTTTTTT46010.189423725352478No Hit
TATCAACGCAGAGTACTTTTTTTTT43930.18086034024634554No Hit
GTCCTACAGTGTGCATTTCTCATTT37160.15298816853071248No Hit
CTGAAGGACCTGGAATATGGCGAGA31000.1276273741779356No Hit
CTGTAGGACCTGGAATATGGCGAGA26560.10934784058599903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCC400.00528505814.2480389
GTGTCGT400.005285780414.24774511
TAGGACC10550.014.1471494
GGTATCA13400.013.2797061
TGTCGAG905.4439624E-712.65996918
GGCGAGG16850.011.66445319
TAAGACG907.408449E-611.6190854
ACCGTGC907.4767304E-611.6095128
TGTAGGA38250.011.30911352
AGGACGT38550.011.0977965
GGACGTG37800.010.9815196
CTGTAGG37300.010.9701181
AATGTCC8100.010.9059068
TTAGGAC22300.010.8704643
AGGACCG1053.4372715E-610.8652715
TGGCGAG36250.010.81772418
GACGTGG18200.010.8034587
AGGACCT22250.010.7676065
GTAGGAC38500.010.7409153
GTGGTAT4900.010.6811561
Produced by FastQC (version 0.11.5)