FastQCFastQC Report
Thu 26 May 2016
SRR1512394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141484
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45803.237115150829776No Hit
GGTATCAACGCAGAGTACTTTTTTT35022.475191541092986No Hit
TATCAACGCAGAGTACTTTTTTTTT29612.0928161488224815No Hit
GTACTGGTTCACTATCGGTCAGTCA16711.1810522744621301No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14731.0411071216533319No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12810.9054027310508609No Hit
GGATACCACGTGTCCCGCCCTACTC10110.714568431766136No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9920.7011393514461No Hit
ATACAGGGTGACAGCCCCGTACACA8580.606428995504792No Hit
ATCATTAACTGAATCCATAGGTTAA8530.6028950269995194No Hit
GTATTTAGCCTTGGAGGATGGTCCC7530.5322156568940657No Hit
GTACATGGGGGTTAAGCGACTAAGC7040.4975827655423935No Hit
GTGGTATCAACGCAGAGTACATGGG6850.48415368522235736No Hit
GGTTAATGAGGCGAACCGGGGGAAC6480.4580023182833394No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6340.44810720646857594No Hit
GGTATCAACGCAGAGTACATGGGGG5790.40923355291057645No Hit
GTTCACTATCGGTCAGTCAGGAGTA5760.40711317180741285No Hit
CCCATATTCAGACAGGATACCACGT5640.3986316473947584No Hit
CTATCGGTCAGTCAGGAGTATTTAG5580.3943908851884312No Hit
GTGGTATCAACGCAGAGTACTTTTT5210.36823951824941337No Hit
GTGGATGCCCTGGCAGTCAGAGGCG5170.36541234344519524No Hit
TATTCAGACAGGATACCACGTGTCC5140.3632919623420316No Hit
GTTAATGATAGTGTGTCGAAACACA5140.3632919623420316No Hit
GTCAGGAGTATTTAGCCTTGGAGGA4890.3456221198156682No Hit
GCCTTGGAGGATGGTCCCCCCATAT4860.34350173871250456No Hit
GTATCAACGCAGAGTACATGGGGGT4780.3378473891040683No Hit
TCTAAGTACCCCGAGGAAAAGAAAT4720.3336066268977411No Hit
ACTTAGATGTTTCAGTTCCCCCGGT4720.3336066268977411No Hit
CGGTACTGGTTCACTATCGGTCAGT4680.33077945209352294No Hit
ACCCTGTATCGCGCGCCTTTCCAGA4630.3272454835882503No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4380.30957564106188684No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4150.2933193859376325No Hit
AGCGTACACGGTGGATGCCCTGGCA4100.28978541743235986No Hit
TCACGGTACTGGTTCACTATCGGTC4070.2876650363291962No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4050.28625144892708715No Hit
GGTTCACTATCGGTCAGTCAGGAGT3900.27564954341126907No Hit
ATCAACGCAGAGTACTTTTTTTTTT3840.2714087812049419No Hit
CATCTAAGTACCCCGAGGAAAAGAA3660.2586864945859602No Hit
GTTATAACCGGCGATTTCCGAATGG3490.24667100166803313No Hit
GTACTTAGATGTTTCAGTTCCCCCG3390.2396030646574878No Hit
GTGCTAATCTGCGATAAGCGTCGGT3380.23889627095643323No Hit
ACGCAGAGTACATGGGGGTTAAGCG3340.23606909615221508No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3130.22122642843006982No Hit
GGAGTATTTAGCCTTGGAGGATGGT3080.21769245992479713No Hit
GGTGATATGAACCGTTATAACCGGC3070.21698566622374263No Hit
GATATGAACCGTTATAACCGGCGAT3060.21627887252268807No Hit
GGTATCAACGCAGAGTACATGGGGA3040.21486528512057898No Hit
TATCAACGCAGAGTACATGGGGGTT2990.21133131661530635No Hit
GTCCCGCCCTACTCATCGAGCTCAC2960.2092109355121427No Hit
GAGTACATGGGGGTTAAGCGACTAA2870.20284979220265187No Hit
GGGTGACAGCCCCGTACACAAAAAT2870.20284979220265187No Hit
GCCCTACTCATCGAGCTCACAGCAT2640.18659353707839754No Hit
CCCCAGTAGCGGCGAGCGAACGGGG2590.18305956857312486No Hit
TCATTAACCTATGGATTCAGTTAAT2570.1816459811710158No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG2520.1781120126657431No Hit
ATCCATAGGTTAATGAGGCGAACCG2460.1738712504594159No Hit
CTATCATTAACTGAATCCATAGGTT2440.17245766305730684No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2420.17104407565519777No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2320.1639761386446524No Hit
GTGGTATCCTGTCTGAATATGGGGG2320.1639761386446524No Hit
CACTATCATTAACTGAATCCATAGG2300.16256255124254335No Hit
CACACACACTGATTCAGGCTCTGGG2290.1618557575414888No Hit
GGGTACTTAGATGTTTCAGTTCCCC2230.15761499533516157No Hit
TAAGTACCCCGAGGAAAAGAAATCA2130.1505470583246162No Hit
AGCTCACAGCATGTGCATTTTTGTG2110.14913347092250714No Hit
GGAGGATGGTCCCCCCATATTCAGA2060.14559950241723446No Hit
CCGTTATAACCGGCGATTTCCGAAT2030.14347912131407087No Hit
GAATATGGGGGGACCATCCTCCAAG2030.14347912131407087No Hit
TTTGTGTACGGGGCTGTCACCCTGT2030.14347912131407087No Hit
GGTATCAACGCAGAGTACATGGGCA2020.1427723276130163No Hit
NTATCAACGCAGAGTACTTTTTTTT2010.14206553391196178No Hit
TCGGTAAGGTGATATGAACCGTTAT2000.14135874021090725No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2000.14135874021090725No Hit
GTCCCCCCATATTCAGACAGGATAC1970.13923835910774365No Hit
GATTCAGGCTCTGGGCTGCTCCCCG1970.13923835910774365No Hit
CCTATGGATTCAGTTAATGATAGTG1950.13782477170563454No Hit
GGGTTTCCCCATTCGGAAATCGCCG1900.13429080320036188No Hit
AGTCAGGAGTATTTAGCCTTGGAGG1890.13358400949930732No Hit
ATTTAGCCTTGGAGGATGGTCCCCC1860.13146362839614373No Hit
GCGTACACGGTGGATGCCCTGGCAG1860.13146362839614373No Hit
TTCCAGACGCTTCCACTAACACACA1850.1307568346950892No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1800.1272228661898165No Hit
GGGAAACCCAGTGTGTTTCGACACA1760.12439569138559838No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT1730.12227531028243475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1730.12227531028243475No Hit
CCCTGGCAGTCAGAGGCGATGAAGG1720.12156851658138024No Hit
GTACGGGGCTGTCACCCTGTATCGC1710.12086172288032569No Hit
GTACATGGGCATCTAAGTACCCCGA1690.11944813547821662No Hit
GTGCATTTTTGTGTACGGGGCTGTC1660.11732775437505302No Hit
CAGTAGCGGCGAGCGAACGGGGAGC1630.1152073732718894No Hit
AAGTACCCCGAGGAAAAGAAATCAA1620.11450057957083486No Hit
GTATCAACGCAGAGTACATGGGGAA1610.11379378586978033No Hit
GTCTGAATATGGGGGGACCATCCTC1590.11238019846767126No Hit
GGGCTGTCACCCTGTATCGCGCGCC1580.1116734047666167No Hit
CAACGCAGAGTACTTTTTTTTTTTT1550.10955302366345311No Hit
CATTAACTGAATCCATAGGTTAATG1530.10813943626134404No Hit
GTATCGCGCGCCTTTCCAGACGCTT1520.10743264256028949No Hit
GAGTAGGGCGGGACACGTGGTATCC1520.10743264256028949No Hit
CGTGGTATCCTGTCTGAATATGGGG1500.10601905515818043No Hit
GGGGAAACCCAGTGTGTTTCGACAC1490.1053122614571259No Hit
GGATGGTCCCCCCATATTCAGACAG1470.10389867405501682No Hit
NGTATCAACGCAGAGTACTTTTTTT1470.10389867405501682No Hit
GATCAACGCAGAGTACTTTTTTTTT1460.10319188035396228No Hit
CACTGATTCAGGCTCTGGGCTGCTC1450.10248508665290776No Hit
ATGATAGTGTGTCGAAACACACTGG1450.10248508665290776No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC1440.10177829295185321No Hit
GTACACAAAAATGCACATGCTGTGA1420.10036470554974414No Hit
TATTTAGCCTTGGAGGATGGTCCCC1420.10036470554974414No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1420.10036470554974414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCA250.00607207918.96372613
TGTATTC250.00608457118.95698512
GATCAAC401.9351685E-417.3975581
CGAATGG456.8431074E-414.73908319
GGTATCA7250.014.5351031
CCGAATG500.001521520913.26046218
CTACTGG450.00847028613.2552831
ACCTATG604.116887E-412.6424847
TTAACTG2000.012.3264225
TGAATCC1850.012.29205310
GAATCCA1900.011.97283411
GTGGTAT2750.011.9297541
TAACTGA2000.011.8523286
AATCCAT1950.011.66583712
CCCCCGG1406.85759E-1011.50142118
ACTGAAT2000.011.3419358
GTGTCGA1451.204171E-911.11666713
TGTCGAA1451.204171E-911.11666714
CCCCGGT1451.2187229E-911.10876619
TACTGGT4050.010.9997332
Produced by FastQC (version 0.11.5)