FastQCFastQC Report
Thu 26 May 2016
SRR1512394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141484
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53893.8089112549828954No Hit
GGTATCAACGCAGAGTACTTTTTTT42573.0088207853891604No Hit
TATCAACGCAGAGTACTTTTTTTTT37712.665319046676656No Hit
GTACTGGTTCACTATCGGTCAGTCA19361.3683526052415822No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17941.267987899691838No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13800.9753753074552599No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11360.8029176443979531No Hit
GGATACCACGTGTCCCGCCCTACTC11300.7986768821916259No Hit
ATACAGGGTGACAGCCCCGTACACA10430.7371858301998813No Hit
ATCATTAACTGAATCCATAGGTTAA10070.7117412569619179No Hit
GGTTAATGAGGCGAACCGGGGGAAC8060.5696757230499562No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7990.5647281671425745No Hit
GTATTTAGCCTTGGAGGATGGTCCC7770.5491787057193745No Hit
GTGGTATCAACGCAGAGTACATGGG7540.5329224505951203No Hit
GTACATGGGGGTTAAGCGACTAAGC7370.5209069576771932No Hit
GTGGTATCAACGCAGAGTACTTTTT7070.4997031466455571No Hit
CCCATATTCAGACAGGATACCACGT7030.4968759718413389No Hit
GGTATCAACGCAGAGTACATGGGGG6780.4792061293149756No Hit
TATTCAGACAGGATACCACGTGTCC6320.4466936190664668No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6310.44598682536541234No Hit
CTATCGGTCAGTCAGGAGTATTTAG6250.44174606315908516No Hit
GTTCACTATCGGTCAGTCAGGAGTA6000.4240762206327217No Hit
TCTAAGTACCCCGAGGAAAAGAAAT5740.40569958440530374No Hit
GCCTTGGAGGATGGTCCCCCCATAT5660.40004523479686743No Hit
GTATCAACGCAGAGTACATGGGGGT5570.39368409148737665No Hit
CGGTACTGGTTCACTATCGGTCAGT5520.390150122982104No Hit
ACTTAGATGTTTCAGTTCCCCCGGT5300.3746006615589042No Hit
GTTAATGATAGTGTGTCGAAACACA5270.37248028045574055No Hit
GTCAGGAGTATTTAGCCTTGGAGGA5260.3717734867546861No Hit
AGCGTACACGGTGGATGCCCTGGCA5260.3717734867546861No Hit
TCACGGTACTGGTTCACTATCGGTC5010.35410364422832263No Hit
ACCCTGTATCGCGCGCCTTTCCAGA4890.3456221198156682No Hit
GCCCAGAGCCTGAATCAGTGTGTGT4820.34067456390828643No Hit
GGTTCACTATCGGTCAGTCAGGAGT4800.33926097650617737No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG4800.33926097650617737No Hit
ATCAACGCAGAGTACTTTTTTTTTT4790.33855418280512284No Hit
CATCTAAGTACCCCGAGGAAAAGAA4460.31522999067032315No Hit
GTTATAACCGGCGATTTCCGAATGG4160.29402617963868705No Hit
GCCTGAATCAGTGTGTGTGTTAGTG4030.2848378615249781No Hit
TATCAACGCAGAGTACATGGGGGTT3980.28130389301970543No Hit
GGAGTATTTAGCCTTGGAGGATGGT3960.2798903056175963No Hit
GGTATCAACGCAGAGTACATGGGGA3930.2777699245144327No Hit
ACGCAGAGTACATGGGGGTTAAGCG3810.2692884001017783No Hit
GTACTTAGATGTTTCAGTTCCCCCG3690.26080687568912386No Hit
GAATCAGTGTGTGTGTTAGTGGAAG3580.25303214497752397No Hit
GTGCTAATCTGCGATAAGCGTCGGT3460.2445506205648695No Hit
GATATGAACCGTTATAACCGGCGAT3460.2445506205648695No Hit
TCATTAACCTATGGATTCAGTTAAT3400.24030985835854232No Hit
CCCCAGTAGCGGCGAGCGAACGGGG3400.24030985835854232No Hit
GAGTACATGGGGGTTAAGCGACTAA3170.22405360323428797No Hit
ATCCATAGGTTAATGAGGCGAACCG3150.2226400158321789No Hit
GGGTGACAGCCCCGTACACAAAAAT3130.22122642843006982No Hit
GTGTTAGTGGAAGCGTCTGGAAAGG3080.21769245992479713No Hit
GCCCTACTCATCGAGCTCACAGCAT3000.21203811031636086No Hit
CACTATCATTAACTGAATCCATAGG2900.20497017330581552No Hit
GTCCCGCCCTACTCATCGAGCTCAC2880.20355658590370643No Hit
GGTGATATGAACCGTTATAACCGGC2850.2014362048005428No Hit
CCGTTATAACCGGCGATTTCCGAAT2660.18800712448050663No Hit
TCGGTAAGGTGATATGAACCGTTAT2650.1873003307794521No Hit
CTATCATTAACTGAATCCATAGGTT2620.18517994967628848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2540.1795256000678522No Hit
CACACACACTGATTCAGGCTCTGGG2490.17599163156257952No Hit
CAGTAGCGGCGAGCGAACGGGGAGC2480.175284837861525No Hit
GTGGTATCCTGTCTGAATATGGGGG2460.1738712504594159No Hit
AGTCAGGAGTATTTAGCCTTGGAGG2420.17104407565519777No Hit
TAAGTACCCCGAGGAAAAGAAATCA2420.17104407565519777No Hit
GAATATGGGGGGACCATCCTCCAAG2410.17033728195414322No Hit
GGGTACTTAGATGTTTCAGTTCCCC2400.16963048825308868No Hit
GTGTGTTAGTGGAAGCGTCTGGAAA2380.16821690085097962No Hit
TTTGTGTACGGGGCTGTCACCCTGT2370.16751010714992506No Hit
CCTATGGATTCAGTTAATGATAGTG2370.16751010714992506No Hit
AGCTCACAGCATGTGCATTTTTGTG2360.16680331344887056No Hit
GTGTGTGTTAGTGGAAGCGTCTGGA2340.16538972604676147No Hit
GGTATCAACGCAGAGTACATGGGCA2320.1639761386446524No Hit
GCGTACACGGTGGATGCCCTGGCAG2140.15125385202567074No Hit
GAGTAGGGCGGGACACGTGGTATCC2130.1505470583246162No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT2100.14842667722145259No Hit
AACGCAGAGTACTTTTTTTTTTTTT2070.146306296118289No Hit
ATTTAGCCTTGGAGGATGGTCCCCC2060.14559950241723446No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2060.14559950241723446No Hit
GGGAAACCCAGTGTGTTTCGACACA2060.14559950241723446No Hit
TTCCAGACGCTTCCACTAACACACA2050.14489270871617993No Hit
GTACATGGGCATCTAAGTACCCCGA2050.14489270871617993No Hit
TCCCCCAGTAGCGGCGAGCGAACGG1980.13994515280879816No Hit
GGGTTTCCCCATTCGGAAATCGCCG1970.13923835910774365No Hit
CATTAACTGAATCCATAGGTTAATG1920.13570439060247094No Hit
GTATCGCGCGCCTTTCCAGACGCTT1910.1349975969014164No Hit
GATTCAGGCTCTGGGCTGCTCCCCG1910.1349975969014164No Hit
GGTATCAACGCAGAGTACATGGGAT1900.13429080320036188No Hit
AAGTACCCCGAGGAAAAGAAATCAA1900.13429080320036188No Hit
GTCCCCCCATATTCAGACAGGATAC1880.13287721579825282No Hit
TAATGATAGTGTGTCGAAACACACT1880.13287721579825282No Hit
GGGGTTAAGCGACTAAGCGTACACG1850.1307568346950892No Hit
GGAGGATGGTCCCCCCATATTCAGA1850.1307568346950892No Hit
AGGTTAATGAGGCGAACCGGGGGAA1850.1307568346950892No Hit
GTGCATTTTTGTGTACGGGGCTGTC1840.13005004099403467No Hit
CGTGGTATCCTGTCTGAATATGGGG1840.13005004099403467No Hit
CAACGCAGAGTACTTTTTTTTTTTT1810.12792965989087104No Hit
GTATCAACGCAGAGTACATGGGGAA1810.12792965989087104No Hit
CCCTGGCAGTCAGAGGCGATGAAGG1790.12651607248876198No Hit
ATGATAGTGTGTCGAAACACACTGG1790.12651607248876198No Hit
CCACGTGTCCCGCCCTACTCATCGA1740.1229821039834893No Hit
ATTCAGACAGGATACCACGTGTCCC1730.12227531028243475No Hit
CACTGATTCAGGCTCTGGGCTGCTC1730.12227531028243475No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT1710.12086172288032569No Hit
GTCTGAATATGGGGGGACCATCCTC1690.11944813547821662No Hit
TCAACGCAGAGTACTTTTTTTTTTT1640.11591416697294395No Hit
CTACTGGGGGAATCTCGGTTGATTT1640.11591416697294395No Hit
AAAATGCACATGCTGTGAGCTCGAT1630.1152073732718894No Hit
GTATCAACGCAGAGTACATGGGGAG1630.1152073732718894No Hit
GTATCAACGCAGAGTACATGGGCAT1630.1152073732718894No Hit
CATTAACCTATGGATTCAGTTAATG1600.11308699216872578No Hit
CCCTCACGGTACTGGTTCACTATCG1600.11308699216872578No Hit
TATTTAGCCTTGGAGGATGGTCCCC1560.11025981736450764No Hit
GAGCAGCCCAGAGCCTGAATCAGTG1560.11025981736450764No Hit
GCTAAATACTCCTGACTGACCGATA1540.10884622996239857No Hit
GGTATCAACGCAGAGTACATGGGCG1540.10884622996239857No Hit
CTCCAAGGCTAAATACTCCTGACTG1540.10884622996239857No Hit
GGATGGTCCCCCCATATTCAGACAG1530.10813943626134404No Hit
GCATGTGCATTTTTGTGTACGGGGC1530.10813943626134404No Hit
GCTCTGGGCTGCTCCCCGTTCGCTC1530.10813943626134404No Hit
GGGCTGTCACCCTGTATCGCGCGCC1530.10813943626134404No Hit
GGGGAAACCCAGTGTGTTTCGACAC1520.10743264256028949No Hit
CAGGGTGACAGCCCCGTACACAAAA1510.10672584885923497No Hit
GGGCGGGACACGTGGTATCCTGTCT1510.10672584885923497No Hit
CTGTGAGCTCGATGAGTAGGGCGGG1500.10601905515818043No Hit
CTGTCACCCTGTATCGCGCGCCTTT1500.10601905515818043No Hit
CACGTGTCCCGCCCTACTCATCGAG1450.10248508665290776No Hit
AACGGGGAGCAGCCCAGAGCCTGAA1430.10107149925079868No Hit
GTGTTTCGACACACTATCATTAACT1430.10107149925079868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.014.4580251
CGAATGG707.163986E-613.56915719
TGTCCCG2050.013.43677411
GTGGTAT3250.012.8775931
TGGTATC3250.012.8684832
CCGAATG751.4587376E-512.66454518
GTGTCCC2200.012.52063210
TTAACTG2250.012.2467245
ATCCATA2100.012.2122413
TAACTGA2200.012.0888856
CGCCCTA2300.011.97625516
TGAATCC2150.011.92823510
TCCCGCC2350.011.72144113
TGTCTGA657.955781E-411.69035110
AATCCAT2300.011.56328112
ACTGAAT2400.011.4772448
CCGCCCT2400.011.47724415
GTCCCGC2400.011.47724412
TCCATAG2250.011.39809114
CATAGGT2500.011.3980916
Produced by FastQC (version 0.11.5)