FastQCFastQC Report
Thu 26 May 2016
SRR1512393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768972
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58000.327874042099027No Hit
GTCCTAAAGTGTGTATTTCTCATTT41410.2340907600572536No Hit
GTCCTACAGTGGACATTTCTAAATT41160.23267750987579225No Hit
GGTATCAACGCAGAGTACTTTTTTT38870.21973213821360654No Hit
CTGTAGGACGTGGAATATGGCAAGA37980.21470096756760426No Hit
CTTTAGGACGTGAAATATGGCGAGG35000.19785502540458524No Hit
TATCAACGCAGAGTACTTTTTTTTT34450.19474587500537036No Hit
GTCCTACAGTGTGCATTTCTCATTT23350.13199756694848758No Hit
CTGAAGGACCTGGAATATGGCGAGA18190.10282808320312589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG350.002194405816.2588634
TAGGACC7500.013.6574464
GGTATCA11500.013.2216941
CTAGGAC2450.012.0005893
ACCGGGG1001.9598156E-611.38541115
TAAGACT1751.8189894E-1211.3812044
CGTGCGC752.1189614E-411.37376810
GTGCTAG1155.362526E-711.1611711
AATGTCC7150.010.975478
GTCTAAT1103.8134822E-610.770921
GTGTAGG3300.010.770921
GGCGAGG11550.010.74857119
GTGGTAT4150.010.7060351
TGGTATC3900.010.7002772
AACCGGG803.8160873E-410.67382314
AAATGTC7650.010.539057
TGTAGGA25200.010.1994262
GTGTTAC1655.4296834E-910.1725351
GACGTGG11200.010.1626567
GTAGGAC25250.010.0665443
Produced by FastQC (version 0.11.5)