FastQCFastQC Report
Thu 26 May 2016
SRR1512393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768972
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70790.4001759213825883No Hit
GGTATCAACGCAGAGTACTTTTTTT49050.27727968560271166No Hit
TATCAACGCAGAGTACTTTTTTTTT47250.2671042842961901No Hit
CTGTAGGACGTGGAATATGGCAAGA44780.2531413725033522No Hit
GTCCTAAAGTGTGTATTTCTCATTT44670.25251954242350927No Hit
GTCCTACAGTGGACATTTCTAAATT44370.25082364220575565No Hit
CTTTAGGACGTGAAATATGGCGAGG43130.24381392130570748No Hit
GTCCTACAGTGTGCATTTCTCATTT26370.1490696291405404No Hit
CTGAAGGACCTGGAATATGGCGAGA23250.13143226687590306No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22810.12894494655653113No Hit
CTGTAGGACCTGGAATATGGCGAGA20230.11436020468385028No Hit
ATTTAGAAATGTCCACTGTAGGACG19190.10848108392897118No Hit
TTTCTAAATTTTCCACCTTTTTCAG18500.10458051342813793No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17720.1001711728619786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1101.2732926E-1114.67487318
TGGCGAA653.3893612E-614.6084718
GGTATCA12700.014.0826131
CCAACGA903.662535E-813.71572919
TAGGACC8500.012.5273064
CGTGCGC550.003073113712.08860710
AATGTCC6900.011.7006688
AAATGTC6850.011.5087567
CATTCGT752.0832603E-411.39460619
GGACCTG15250.011.3355456
GGCGAGG11150.011.24131619
AGGACCT16150.011.1262265
CCACCTT7300.011.05859813
CACCTTT7500.011.01509814
CTCCTAT1657.8216544E-1110.9546561
ACCTTTT7450.010.83379815
TAGAAAT7600.010.7582954
CTATACC1951.8189894E-1210.7262184
ATTTAGA7650.010.6946391
ACATTCG803.7877567E-410.68244318
Produced by FastQC (version 0.11.5)