FastQCFastQC Report
Thu 26 May 2016
SRR1512392_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512392_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564537
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT87340.3405682975133523No Hit
CTGTAGGACGTGGAATATGGCAAGA78980.3079698206732833No Hit
GTCCTAAAGTGTGTATTTCTCATTT72340.2820782074893051No Hit
GTATCAACGCAGAGTACTTTTTTTT63050.24585334506774517No Hit
CTTTAGGACGTGAAATATGGCGAGG57200.22304220995836674No Hit
GTCCTACAGTGTGCATTTCTCATTT54940.2142297030614103No Hit
GGTATCAACGCAGAGTACTTTTTTT38150.14875979562782676No Hit
TATCAACGCAGAGTACTTTTTTTTT35220.13733473137646288No Hit
CTGTAGGACCTGGAATATGGCGAGA35030.13659385690282497No Hit
CTGAAGGACCTGGAATATGGCGAGA32280.12587067373174965No Hit
GTCCTTCAGTGTGCATTTCTCATTT28940.11284688035306178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14650.014.1785854
GGTATCA11900.013.51468851
TCGCGAG500.00153019313.26487718
GTGTAGG4800.013.1554011
CGTGCGC1052.0600055E-812.63792110
TGCGATA604.172829E-412.63792110
GCGCCAC1155.4533302E-912.37239813
TCCAACG1850.012.29177818
CACCTTT10700.011.88300514
AGTGCCG802.9355037E-511.8480510
CGACTAA1451.0004442E-1011.77788217
ACCTTTT10700.011.70564715
GGACCTG28000.011.6870396
AGGACCT29450.011.4978825
AATACCG752.1055885E-411.3819365
GCGATAA752.1182778E-411.37457511
TGTAGGA47150.011.2046922
TCGAACT1105.1361167E-711.1991619
GTACCGT855.4113538E-511.158986
CCACCTT10450.011.07398713