FastQCFastQC Report
Thu 26 May 2016
SRR1512392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512392_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564537
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA94000.36653789748402926No Hit
GTCCTACAGTGGACATTTCTAAATT90120.35140846086447575No Hit
GTCCTAAAGTGTGTATTTCTCATTT81630.31830306991086504No Hit
GTATCAACGCAGAGTACTTTTTTTT74870.29194353600669437No Hit
CTTTAGGACGTGAAATATGGCGAGG69450.2708091168113387No Hit
GTCCTACAGTGTGCATTTCTCATTT59360.23146478292182956No Hit
GGTATCAACGCAGAGTACTTTTTTT50020.1950449535335228No Hit
TATCAACGCAGAGTACTTTTTTTTT48980.1909896406251889No Hit
CTGTAGGACCTGGAATATGGCGAGA42660.16634581602839033No Hit
CTGAAGGACCTGGAATATGGCGAGA39240.15301007550290754No Hit
GTCCTTCAGTGTGCATTTCTCATTT29950.11678521308134762No Hit
ATTTAGAAATGTCCACTGTAGGACG29570.11530346413407176No Hit
GAATATGGCAAGAAAACTGAAAATC27450.10703686474400642No Hit
TTTCTAAATTTTCCACCTTTTTCAG26170.10204571039528773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15650.013.1823244
GGTATCA13900.013.076691
AGAACCG604.067365E-412.6763385
TATGTCG2100.012.20716616
GTCGAGG1459.822543E-1111.7862319
TGTAGGA50050.011.6824682
TGTCGAG2150.011.48167518
TAGACGT752.0605346E-411.4084824
CTTTTTC12500.011.39335517
CTGTAGG50050.011.2373181
CCACCTT11600.011.21525613
TCCAACG1701.2732926E-1111.16995618
ATGTCGA2550.011.16995517
GGCGAGG19950.011.13636119
GTACCGC600.005881601511.0822756
ATCCGTA600.005881601511.0822757
ACCTTTT11600.010.96795615
AGGACCT29650.010.9662085
GTAGGAC52100.010.8756473
GTCCTAC46900.010.8557591
Produced by FastQC (version 0.11.5)