FastQCFastQC Report
Thu 26 May 2016
SRR1512391_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512391_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410912
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76070.31552375200753907No Hit
CTGTAGGACGTGGAATATGGCAAGA70060.29059542612919925No Hit
GTATCAACGCAGAGTACTTTTTTTT65240.27060299173093005No Hit
GTCCTAAAGTGTGTATTTCTCATTT61010.2530577640328639No Hit
CTTTAGGACGTGAAATATGGCGAGG52170.21639114160948222No Hit
GTCCTACAGTGTGCATTTCTCATTT43100.178770523353818No Hit
GGTATCAACGCAGAGTACTTTTTTT39100.1621792914880344No Hit
TATCAACGCAGAGTACTTTTTTTTT36420.1510631661379594No Hit
CTGAAGGACCTGGAATATGGCGAGA28940.12003756254894413No Hit
GTCCTTCAGTGTGCATTTCTCATTT25970.10771857288859983No Hit
CTGTAGGACCTGGAATATGGCGAGA25650.10639127433933716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCAC250.00609259518.9676973
TAGGACC13700.013.9831934
TATACCG500.001519078313.2776645
GGTATCA13050.013.160851
AGGACCT25200.011.9679635
GTGTAGG5000.011.8447651
GTCCTAC38050.011.5957291
TAGAAAT9450.011.4405944
GTATTAG2850.011.4291591
GGACCTG25050.011.3962366
AAATGTC8950.011.3387057
TCCTACA42550.011.2337472
AATGTCC9100.011.219998
GTCCTAA18200.011.1722591
TGCACCG855.4173677E-511.1577015
GACGTGG20650.011.1147067
GGCGAGG16050.011.10278719
CTAGGAC3850.011.0850173
CACCGCG600.005950290711.0649517
GAAATGT9850.010.9766886