FastQCFastQC Report
Thu 26 May 2016
SRR1512391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512391_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410912
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA85280.35372506337850573No Hit
GTCCTACAGTGGACATTTCTAAATT80830.3352673179278215No Hit
GTATCAACGCAGAGTACTTTTTTTT71930.298351827026453No Hit
GTCCTAAAGTGTGTATTTCTCATTT66060.2740041942634157No Hit
CTTTAGGACGTGAAATATGGCGAGG61610.2555464488127315No Hit
GGTATCAACGCAGAGTACTTTTTTT47740.19801635231812692No Hit
GTCCTACAGTGTGCATTTCTCATTT46600.1932878512363786No Hit
TATCAACGCAGAGTACTTTTTTTTT45100.18706613928670976No Hit
CTGAAGGACCTGGAATATGGCGAGA34820.14442667339164597No Hit
CTGTAGGACCTGGAATATGGCGAGA32160.1333935042008999No Hit
GTCCTTCAGTGTGCATTTCTCATTT26210.10871404680054685No Hit
ATTTAGAAATGTCCACTGTAGGACG24750.10265824716953584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14550.013.3302454
GATCACG500.001502189113.29738511
GGTATCA14900.013.0263171
ATTGGAC1850.012.3341853
GCACCGC658.035285E-411.6907376
ACTTCGT658.038083E-411.6902528
TGTAGGA44500.011.2379652
GGCGAGG16500.011.16356519
CTGTAGG43800.011.0565951
GTAGGAC44850.010.8961133
ATCCCGT1408.381903E-910.85523410
AGCGTTG803.7445215E-410.6960515
AGGACCT25300.010.67256455
GAAATGT9100.010.6469226
ACTGTTC6350.010.6201798
GGACCTG25000.010.4485966
AGGACGT42750.010.3638415
GTCCTAC41300.010.3206181
GGACGTG42000.010.2902846
CAAGTCG3000.010.1319726
Produced by FastQC (version 0.11.5)