FastQCFastQC Report
Thu 26 May 2016
SRR1512390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964518
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62480.31804239004173035No Hit
GTCCTAAAGTGTGTATTTCTCATTT57580.2930998850608648No Hit
GTATCAACGCAGAGTACTTTTTTTT55390.2819521124265596No Hit
CTGTAGGACGTGGAATATGGCAAGA55110.280526826427653No Hit
CTTTAGGACGTGAAATATGGCGAGG47770.2431639720277442No Hit
GTCCTACAGTGTGCATTTCTCATTT37490.1908356146393161No Hit
GGTATCAACGCAGAGTACTTTTTTT35120.17877158671999951No Hit
TATCAACGCAGAGTACTTTTTTTTT31150.15856306737835946No Hit
CTGTAGGACCTGGAATATGGCGAGA25760.1311263118994074No Hit
CTGAAGGACCTGGAATATGGCGAGA25100.12776670918769897No Hit
GTCCTTCAGTGTGCATTTCTCATTT22650.11529545669726621No Hit
ATTTAGAAATGTCCACTGTAGGACG22400.11402287991252816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGA400.005362539614.21661410
GGTATCA11850.013.5791861
GTACTAG954.4699846E-813.5090331
TAGGACC11150.013.4396284
TGCGATA604.1746438E-412.63699110
GGCGAGG15100.012.23978219
CTAGGAC3650.011.4331213
TAGGACA9500.011.3811524
TTGGCGA600.00592788311.070394517
AATCCCG2400.011.0576519
AGGACCT22250.010.9975195
ATAGGAC2850.010.9818143
AAATGTC9350.010.955127
ACTGTTC5100.010.9336798
GGACCTG21350.010.75007256
CCCGTTT1159.0625326E-710.71643712
AATGTCC9450.010.7012978
TTAGGAC23300.010.5019223
ATGTCCA9650.010.48298559
TAGAAAT9500.010.482644
Produced by FastQC (version 0.11.5)