FastQCFastQC Report
Thu 26 May 2016
SRR1512390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512390_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964518
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA67860.3454282424492929No Hit
GTATCAACGCAGAGTACTTTTTTTT66090.33641839881334756No Hit
GTCCTACAGTGGACATTTCTAAATT63090.32114747739649113No Hit
GTCCTAAAGTGTGTATTTCTCATTT62180.3165152979000447No Hit
CTTTAGGACGTGAAATATGGCGAGG57690.29365981884614956No Hit
GGTATCAACGCAGAGTACTTTTTTT44470.22636595846920213No Hit
TATCAACGCAGAGTACTTTTTTTTT42070.21414922133571695No Hit
GTCCTACAGTGTGCATTTCTCATTT39970.20345957634391745No Hit
CTGTAGGACCTGGAATATGGCGAGA33330.16965993694127518No Hit
CTGAAGGACCTGGAATATGGCGAGA31160.15861397044974898No Hit
ATTTAGAAATGTCCACTGTAGGACG26230.13351875625471488No Hit
GTCCTTCAGTGTGCATTTCTCATTT23320.11870596248036414No Hit
TTTCTAAATTTTCCACCTTTTTCAG22610.11509184441170811No Hit
GAATATGGCAAGAAAACTGAAAATC22430.11417558912669673No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21280.10832173591690175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1101.8735591E-1013.81081418
GGTATCA12600.013.5929091
TGGACCG604.0647807E-412.6770145
TAGGACC13850.012.4253044
GGCGAGG17400.011.84138319
GGCGAAA658.0610585E-411.68607319
TGGCGAG37700.011.38384718
TAGAAAT10200.011.0923884
TAAGACG600.005840980411.0923884
CCACCTT8550.010.88474613
GCCGGTT1408.38736E-910.85439311
AAATGTC9650.010.8273757
GCCCTAT803.7169052E-410.7044151
GGCGAGA21450.010.66796919
AGGACCT26200.010.59643655
ATGGCGA41200.010.55504917
GTCCTAA19750.010.5508511
ATTTAGA9250.010.4922571
CCGGTTT1451.45118975E-810.47930312
GTATTAG2100.010.421231
Produced by FastQC (version 0.11.5)