FastQCFastQC Report
Thu 26 May 2016
SRR1512389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913635
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT65000.3396677004757961No Hit
GTATCAACGCAGAGTACTTTTTTTT62660.3274396632586674No Hit
GTCCTAAAGTGTGTATTTCTCATTT59500.3109265873586133No Hit
CTGTAGGACGTGGAATATGGCAAGA58280.30455128590352915No Hit
CTTTAGGACGTGAAATATGGCGAGG51600.26964389760847807No Hit
GGTATCAACGCAGAGTACTTTTTTT40460.21143007940385705No Hit
TATCAACGCAGAGTACTTTTTTTTT35870.18744431409333545No Hit
GTCCTACAGTGTGCATTTCTCATTT34550.1805464469452116No Hit
CTGAAGGACCTGGAATATGGCGAGA28650.14971507105587012No Hit
GTCCTTCAGTGTGCATTTCTCATTT25290.13215686376973665No Hit
CTGTAGGACCTGGAATATGGCGAGA24520.12813310793333108No Hit
ATTTAGAAATGTCCACTGTAGGACG21660.11318772911239604No Hit
GAATATGGCAAGAAAACTGAAAATC20670.10801432875130314No Hit
TTTCTAAATTTTCCACCTTTTTCAG20520.10723048021174361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11600.014.6352751
TAGGACC10700.013.5605494
TAAGACG655.5432924E-513.1310284
GTGTAGG4550.013.0157811
GATCTAC801.9375706E-512.3378771
TCCAACG1251.4733814E-912.12679618
CCCCGTA1353.783498E-1011.95037815
AGGACGG802.918539E-511.8544025
GGCGAGG17350.011.19766119
GTCCTAT3000.011.1863411
ACCTTTT8550.011.09942215
AGGACCT22200.010.9359535
GGACGGG700.001513426510.838316
TGTAGGA38450.010.827742
ATTTCTA24400.010.81238515
AAATGTC9000.010.747997
TCTATAC1951.8189894E-1210.6993573
TATACTG3400.010.5992315
GTATCAA26050.010.5712641
GTAGGAC39350.010.531893
Produced by FastQC (version 0.11.5)