##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512389_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1913635 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.76039944921576 31.0 23.0 33.0 10.0 34.0 2 27.505804398435437 31.0 26.0 34.0 16.0 34.0 3 28.668727317382885 31.0 28.0 34.0 16.0 34.0 4 32.454688590039375 35.0 33.0 37.0 22.0 37.0 5 32.75010229223441 35.0 35.0 37.0 25.0 37.0 6 33.007882903479505 37.0 35.0 37.0 26.0 37.0 7 32.91260768119312 37.0 35.0 37.0 26.0 37.0 8 32.917422078923096 37.0 35.0 37.0 26.0 37.0 9 34.35234514418894 39.0 35.0 39.0 25.0 39.0 10 34.26735140191311 39.0 35.0 39.0 25.0 39.0 11 34.315790106263734 39.0 35.0 39.0 26.0 39.0 12 34.22210557394696 39.0 35.0 39.0 25.0 39.0 13 34.17335385274621 39.0 35.0 39.0 25.0 39.0 14 35.18829870900146 40.0 36.0 41.0 24.0 41.0 15 35.1257178093001 40.0 36.0 41.0 24.0 41.0 16 34.98648070295537 40.0 36.0 41.0 22.0 41.0 17 34.93477648558895 40.0 36.0 41.0 22.0 41.0 18 34.89582705165823 40.0 35.0 41.0 20.0 41.0 19 34.907060123795816 40.0 35.0 41.0 19.0 41.0 20 34.815849940035584 40.0 35.0 41.0 18.0 41.0 21 34.6929012063429 40.0 35.0 41.0 18.0 41.0 22 34.570768197697056 40.0 35.0 41.0 18.0 41.0 23 34.397096102443776 39.0 35.0 41.0 11.0 41.0 24 34.26473700575084 39.0 34.0 41.0 8.0 41.0 25 34.11099608859579 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.174024255421902 1101 2 2.488877183800632 1101 3 1.1708847990990385 1101 4 0.7842897871318399 1101 5 0.5511113730621204 1101 6 0.46423434716012224 1101 7 0.22777602701216182 1101 8 0.03264334821355419 1101 9 -0.19081218204926387 1101 10 -0.12576259097043874 1101 11 -0.08593367399988239 1101 12 0.043578137533458516 1101 13 -0.15342382324827497 1101 14 -0.041557248413020886 1101 15 -0.14715579145965307 1101 16 -0.34122280297498975 1101 17 -0.29357417750939163 1101 18 -0.550355742219466 1101 19 -0.32852745495294045 1101 20 -0.5402836064827596 1101 21 -0.32838225678008115 1101 22 -0.5495857916998546 1101 23 -0.6026215166936737 1101 24 -0.4796734154935507 1101 25 -0.6941170571127273 1102 1 3.305343736468899 1102 2 1.9324437042720035 1102 3 0.7664549365741955 1102 4 0.391177299496114 1102 5 0.26753870604836294 1102 6 0.0398018949207426 1102 7 -0.0958654877236853 1102 8 -0.05285215497818996 1102 9 -0.03071463255321305 1102 10 -0.08273813751034709 1102 11 -0.08152363729793421 1102 12 -0.06174399447858292 1102 13 -0.2076321362319291 1102 14 -0.3105753973688863 1102 15 -0.368535013245058 1102 16 -0.5050055914742941 1102 17 -0.23883959369855035 1102 18 -0.597954373001933 1102 19 -0.4632912026029885 1102 20 -0.5435653443209105 1102 21 -0.6778894856834441 1102 22 -0.6441872040076575 1102 23 -0.5298594034651245 1102 24 -0.5212404829212289 1102 25 -0.6815526433365164 1103 1 2.8858201888675694 1103 2 1.459545747729564 1103 3 0.3640287640474007 1103 4 0.298898671497426 1103 5 -0.10710724922657278 1103 6 7.955329268583E-5 1103 7 -0.07048391545953336 1103 8 -0.2595801217766862 1103 9 -0.32325199887645084 1103 10 -0.5307503769937725 1103 11 -0.5096097389207728 1103 12 -0.5568516934074239 1103 13 -0.42473197645922056 1103 14 -0.7102285298333584 1103 15 -0.9020600635624234 1103 16 -0.7290284023544515 1103 17 -0.931414245457205 1103 18 -0.8177802613171608 1103 19 -1.0443775235217103 1103 20 -0.886119119317172 1103 21 -0.9559106972957494 1103 22 -1.085017375012221 1103 23 -1.0231043713436705 1103 24 -1.1061046504365706 1103 25 -1.2837863798763962 1104 1 3.9265763816211106 1104 2 1.7853004614028372 1104 3 0.7200384474550816 1104 4 0.5066403109900008 1104 5 0.15390449106569548 1104 6 0.058066413923810956 1104 7 0.005498092603062332 1104 8 -0.1210969716863417 1104 9 -0.39159563144821874 1104 10 -0.12142194857705846 1104 11 -0.11888463401471938 1104 12 -0.22397443040225795 1104 13 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0.7417281443134272 1107 11 0.8139749166186547 1107 12 0.6726559568899972 1107 13 0.7206926281511628 1107 14 0.8773897282895646 1107 15 0.6348645102605204 1107 16 0.6941798321919066 1107 17 0.6687981115529453 1107 18 0.6109900679354396 1107 19 0.6957545927662707 1107 20 0.7681503863077666 1107 21 0.5593710688550573 1107 22 0.5831670361614272 1107 23 0.6414471217963253 1107 24 0.5162922205416578 1107 25 0.6195156113331493 1108 1 4.3002990013495825 1108 2 2.1971960244650646 1108 3 1.206758904328968 1108 4 0.7966994088999755 1108 5 0.6399049107445194 1108 6 0.29396911426901795 1108 7 0.18440470583610846 1108 8 0.1466594884187984 1108 9 0.15827894492989714 1108 10 -0.06257845174728516 1108 11 -0.06466720472580079 1108 12 -0.2034451413464069 1108 13 -0.026203522015109115 1108 14 -0.10753372303599207 1108 15 -0.24404330204295377 1108 16 -0.07009151741923603 1108 17 -0.03960464767592953 1108 18 0.002291374202208374 1108 19 -1.1334752110059299E-4 1108 20 -0.12703453035995693 1108 21 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-0.931623599936998 2315 23 -0.889416415113871 2315 24 -0.9231282037353594 2315 25 -0.6203947292300143 2316 1 2.9130837798774714 2316 2 1.2435281726989054 2316 3 -0.05846988072228143 2316 4 -1.135202091114138 2316 5 -1.0068639611309393 2316 6 -1.2565466221147048 2316 7 -1.1937413704142124 2316 8 -1.1869843241192086 2316 9 -1.4440615257595084 2316 10 -1.4993858531023179 2316 11 -1.2471147925736403 2316 12 -1.4347900881415612 2316 13 -1.3564608385290455 2316 14 -1.4122560494233127 2316 15 -1.5003886062374292 2316 16 -1.1948158159176856 2316 17 -1.1470891026382972 2316 18 -0.9509151575178052 2316 19 -0.9112893525738102 2316 20 -1.186255414503563 2316 21 -1.1311175258350588 2316 22 -1.3455334277103859 2316 23 -1.3566306113348645 2316 24 -1.6590516873625916 2316 25 -1.6358183938021114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 98485.0 3 9.0 4 864.0 5 3701.0 6 4404.0 7 3900.0 8 3670.0 9 3602.0 10 3394.0 11 3619.0 12 3510.0 13 3591.0 14 3616.0 15 3662.0 16 3833.0 17 4001.0 18 4600.0 19 5644.0 20 7064.0 21 8448.0 22 10019.0 23 11720.0 24 13645.0 25 15474.0 26 17577.0 27 19858.0 28 22782.0 29 27007.0 30 33124.0 31 41372.0 32 53972.0 33 71167.0 34 97488.0 35 141573.0 36 229688.0 37 304353.0 38 629199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.484426611303356 20.598624909012575 11.876992099042374 24.039956380641698 2 14.007287881802286 20.101360195181204 43.37153537659292 22.5198165464236 3 18.692711640717146 25.27548407665653 29.70542612932897 26.32637815329735 4 11.792990599903895 17.133216708908996 39.2192549646515 31.854537726535614 5 12.414790732581197 38.52712330373044 36.33655495235608 12.721531011332276 6 30.999484834016034 37.73632715971821 18.519745935214736 12.744442071051024 7 27.564408478351833 31.640413974065986 22.753453590142993 18.04172395743919 8 24.071964131085608 38.71676119502281 20.778270369433265 16.43300430445831 9 26.60294984879956 14.796850924852281 20.742636638165358 37.857562588182795 10 15.116202356405555 28.826959910108503 35.16716949637094 20.88966823711501 11 33.676429164559295 21.706191013224345 26.417541291397423 18.199838530818937 12 24.227436042319187 24.234296178799244 32.65068865820444 18.88757912067713 13 29.672124793906114 22.45512024150956 25.914197904598694 21.95855705998563 14 19.9605485284004 23.678896867295453 27.92029671361699 28.44025789068716 15 21.903097609989842 33.72074860917322 23.205448204216456 21.17070557662048 16 20.766985263454227 28.10185433204445 30.073047215980893 21.05811318852043 17 20.651729171185927 29.65372456062302 28.874267354872053 20.820278913319 18 20.44158063091777 26.922035400408657 32.61058241020916 20.025801558464416 19 23.43674475284058 26.49724517257144 28.109661036565026 21.956349038022953 20 23.662725122032192 27.46784644208485 29.15865074968242 19.710777686200544 21 22.085022575609962 26.813486700818167 27.687178520171386 23.414312203400485 22 22.990716726197686 29.47312296418859 27.740772954306074 19.795387355307646 23 20.986545486385833 28.98107790258284 29.23626327244433 20.796113338587 24 23.23884194983418 26.61599030922118 29.924682604454468 20.220485136490176 25 21.90691335534675 28.114402635736717 29.456654600714515 20.522029408202016 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1738.0 1 1738.0 2 1527.5 3 1317.0 4 1317.0 5 1317.0 6 2365.0 7 3413.0 8 3413.0 9 3413.0 10 4217.0 11 5021.0 12 5021.0 13 5021.0 14 7360.5 15 9700.0 16 9700.0 17 9700.0 18 16928.5 19 24157.0 20 24157.0 21 24157.0 22 39606.5 23 55056.0 24 55056.0 25 55056.0 26 82272.5 27 109489.0 28 109489.0 29 109489.0 30 141441.0 31 173393.0 32 173393.0 33 173393.0 34 190190.0 35 206987.0 36 206987.0 37 206987.0 38 216073.5 39 225160.0 40 225160.0 41 225160.0 42 232665.0 43 240170.0 44 240170.0 45 240170.0 46 242729.5 47 245289.0 48 245289.0 49 245289.0 50 229619.0 51 213949.0 52 213949.0 53 213949.0 54 184575.5 55 155202.0 56 155202.0 57 155202.0 58 131659.0 59 108116.0 60 108116.0 61 108116.0 62 88753.0 63 69390.0 64 69390.0 65 69390.0 66 53300.0 67 37210.0 68 37210.0 69 37210.0 70 27266.5 71 17323.0 72 17323.0 73 17323.0 74 12233.0 75 7143.0 76 7143.0 77 7143.0 78 4984.5 79 2826.0 80 2826.0 81 2826.0 82 1908.0 83 990.0 84 990.0 85 990.0 86 678.0 87 366.0 88 366.0 89 366.0 90 249.5 91 133.0 92 133.0 93 133.0 94 97.0 95 61.0 96 61.0 97 61.0 98 48.5 99 36.0 100 36.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.302701403350169 2 0.18890749803384657 3 0.18038967723729968 4 0.16873646228251468 5 0.15143953784290107 6 0.18660820898447195 7 0.46393382228063346 8 0.24476977061978905 9 0.22203816297256268 10 0.1973730622610895 11 0.19193837905347677 12 0.21174361881968087 13 0.22527807026940877 14 0.23275075967987627 15 0.30188620086902673 16 0.5048507160456409 17 0.5092925244364782 18 0.23243722026405245 19 0.21310228962158403 20 0.20343482430034984 21 0.23489327902133897 22 0.3253494004865087 23 0.3341285041295754 24 0.3052828778737847 25 0.34572946251505643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1913635.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.54583869369819 #Duplication Level Percentage of deduplicated Percentage of total 1 87.06444770412841 61.42034483691364 2 8.980204062819878 12.670320545043683 3 2.0155396257614955 4.265637999591818 4 0.6957268349180944 1.9632253228403624 5 0.3358694990186467 1.1847097749951334 6 0.19966504675041097 0.8451322908494519 7 0.1236414116061297 0.6105670955319017 8 0.09464628214536455 0.5341521082548107 9 0.0655918021960845 0.4164505827618541 >10 0.3612516742340489 4.761689215627028 >50 0.03068599538793077 1.5092372945749566 >100 0.027188869473140507 4.026913871825781 >500 0.0030701196483285437 1.5436111950073657 >1k 0.0020966670769072947 2.6788819999730826 >5k 3.7440483516201697E-4 1.5691258662091898 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6500 0.3396677004757961 No Hit GTATCAACGCAGAGTACTTTTTTTT 6266 0.3274396632586674 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5950 0.3109265873586133 No Hit CTGTAGGACGTGGAATATGGCAAGA 5828 0.30455128590352915 No Hit CTTTAGGACGTGAAATATGGCGAGG 5160 0.26964389760847807 No Hit GGTATCAACGCAGAGTACTTTTTTT 4046 0.21143007940385705 No Hit TATCAACGCAGAGTACTTTTTTTTT 3587 0.18744431409333545 No Hit GTCCTACAGTGTGCATTTCTCATTT 3455 0.1805464469452116 No Hit CTGAAGGACCTGGAATATGGCGAGA 2865 0.14971507105587012 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2529 0.13215686376973665 No Hit CTGTAGGACCTGGAATATGGCGAGA 2452 0.12813310793333108 No Hit ATTTAGAAATGTCCACTGTAGGACG 2166 0.11318772911239604 No Hit GAATATGGCAAGAAAACTGAAAATC 2067 0.10801432875130314 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2052 0.10723048021174361 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.225656930396863E-5 2 0.0 0.0 0.0 0.0 5.225656930396863E-5 3 0.0 0.0 0.0 0.0 5.225656930396863E-5 4 0.0 0.0 0.0 0.0 5.225656930396863E-5 5 0.0 0.0 0.0 0.0 5.225656930396863E-5 6 0.0 0.0 0.0 0.0 5.225656930396863E-5 7 0.0 0.0 0.0 0.0 5.225656930396863E-5 8 0.0 0.0 0.0 0.0 5.225656930396863E-5 9 0.0 0.0 0.0 0.0 5.225656930396863E-5 10 0.0 0.0 0.0 0.0 5.225656930396863E-5 11 0.0 0.0 0.0 0.0 5.225656930396863E-5 12 0.0 0.0 0.0 0.0 5.225656930396863E-5 13 0.0 0.0 0.0 0.0 5.225656930396863E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1160 0.0 14.635275 1 TAGGACC 1070 0.0 13.560549 4 TAAGACG 65 5.5432924E-5 13.131028 4 GTGTAGG 455 0.0 13.015781 1 GATCTAC 80 1.9375706E-5 12.337877 1 TCCAACG 125 1.4733814E-9 12.126796 18 CCCCGTA 135 3.783498E-10 11.950378 15 AGGACGG 80 2.918539E-5 11.854402 5 GGCGAGG 1735 0.0 11.197661 19 GTCCTAT 300 0.0 11.186341 1 ACCTTTT 855 0.0 11.099422 15 AGGACCT 2220 0.0 10.935953 5 GGACGGG 70 0.0015134265 10.83831 6 TGTAGGA 3845 0.0 10.82774 2 ATTTCTA 2440 0.0 10.812385 15 AAATGTC 900 0.0 10.74799 7 TCTATAC 195 1.8189894E-12 10.699357 3 TATACTG 340 0.0 10.599231 5 GTATCAA 2605 0.0 10.571264 1 GTAGGAC 3935 0.0 10.53189 3 >>END_MODULE