FastQCFastQC Report
Thu 26 May 2016
SRR1512389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913635
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71290.37253708256799234No Hit
CTGTAGGACGTGGAATATGGCAAGA70010.36584824169708435No Hit
GTATCAACGCAGAGTACTTTTTTTT69180.36151094644485493No Hit
GTCCTAAAGTGTGTATTTCTCATTT66570.34787198185651913No Hit
CTTTAGGACGTGAAATATGGCGAGG62870.32853705121405075No Hit
GGTATCAACGCAGAGTACTTTTTTT46230.24158211989224695No Hit
TATCAACGCAGAGTACTTTTTTTTT44210.2310262928928453No Hit
GTCCTACAGTGTGCATTTCTCATTT39290.20531606079529272No Hit
CTGAAGGACCTGGAATATGGCGAGA33920.17725428307906158No Hit
CTGTAGGACCTGGAATATGGCGAGA30030.1569264776198178No Hit
ATTTAGAAATGTCCACTGTAGGACG27090.14156304624445099No Hit
GTCCTTCAGTGTGCATTTCTCATTT26270.13727800756152556No Hit
TTTCTAAATTTTCCACCTTTTTCAG25420.13283619917068826No Hit
GAATATGGCAAGAAAACTGAAAATC24770.1294395221659303No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21810.11397157765195558No Hit
GGAATATGGCGAGAAAACTGAAAAT20870.10905946013738252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.014.1453961
GGACCGT500.00150165113.2977866
TAGGACC11150.012.8737614
GTACCGT604.0985443E-412.6645586
GTACTAG1405.0931703E-1112.2339961
ACCGCTC550.003072719612.0888978
AGTCGTG550.003072719612.0888977
GACGTGG20050.012.0329097
GGCGAGG17050.011.97403519
GTCCTAG2800.011.8941631
CCCCGTA1459.822543E-1111.7880615
TCCCGCC1800.011.60705513
TCCAACG2150.011.48315818
GTCGAGG1501.7826096E-1011.39482719
CCCGTAC1501.7826096E-1011.39482716
TGTAGGA40750.011.2443452
CCAACGA1451.2278178E-911.13287619
AAGCCCG600.0058472511.0907655
GGACGTG41300.011.0393246
CCGTACA1553.1468517E-1011.02725117
Produced by FastQC (version 0.11.5)