FastQCFastQC Report
Thu 26 May 2016
SRR1512388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913706
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60500.3161405147917183No Hit
GTCCTACAGTGGACATTTCTAAATT57130.2985307042983614No Hit
GTCCTAAAGTGTGTATTTCTCATTT54550.2850490096179873No Hit
CTGTAGGACGTGGAATATGGCAAGA53830.28128667621881315No Hit
CTTTAGGACGTGAAATATGGCGAGG46150.24115511996095534No Hit
GGTATCAACGCAGAGTACTTTTTTT38860.20306149429431689No Hit
TATCAACGCAGAGTACTTTTTTTTT35310.18451110045116648No Hit
GTCCTACAGTGTGCATTTCTCATTT29820.15582330828246346No Hit
CTGAAGGACCTGGAATATGGCGAGA23620.12342543734513034No Hit
GTCCTTCAGTGTGCATTTCTCATTT23110.12076045118738198No Hit
ATTTAGAAATGTCCACTGTAGGACG21200.1107798167534616No Hit
CTGTAGGACCTGGAATATGGCGAGA19850.10372544163001003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACT250.006076428618.9761816
GGTATCA10800.014.2669041
TAGGACC8600.013.3432084
GTGTAGG3450.012.3105721
TACGGCG854.000325E-612.27870616
TGTACCG658.1420055E-411.6721265
GGCGAGG15000.011.62527619
AGGACCG1001.9706622E-611.3803225
CCAACGT1105.1270945E-711.2002419
AATGTCC6900.011.0973468
GTCCTAC29750.011.0556971
GTCCTAG2950.011.0489441
TCCTACA32750.010.7432572
GTATCAA24750.010.7350961
AAATGTC6900.010.7203117
CGGTTTC1355.8164005E-810.53955213
TGTAGGA32450.010.5210992
TTAGGAC22850.010.45894053
TCCAACG1451.5024852E-810.45587318
CCACCTT7650.010.41555713
Produced by FastQC (version 0.11.5)