FastQCFastQC Report
Thu 26 May 2016
SRR1512388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913706
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72760.3802046918387673No Hit
CTGTAGGACGTGGAATATGGCAAGA63800.33338454287126656No Hit
GTCCTACAGTGGACATTTCTAAATT61570.32173176025993544No Hit
GTCCTAAAGTGTGTATTTCTCATTT57740.30171823676155063No Hit
CTTTAGGACGTGAAATATGGCGAGG55530.2901699634113077No Hit
GGTATCAACGCAGAGTACTTTTTTT49160.2568837637547251No Hit
TATCAACGCAGAGTACTTTTTTTTT45950.2401100273500736No Hit
GTCCTACAGTGTGCATTTCTCATTT32130.16789412793814723No Hit
CTGAAGGACCTGGAATATGGCGAGA31730.16580394271638382No Hit
ATTTAGAAATGTCCACTGTAGGACG24890.1300617754242292No Hit
GTCCTTCAGTGTGCATTTCTCATTT24280.12687424296104No Hit
TTTCTAAATTTTCCACCTTTTTCAG23290.12170103453717553No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23030.12034241414302929No Hit
CTGTAGGACCTGGAATATGGCGAGA22470.11741615483256049No Hit
GAATATGGCAAGAAAACTGAAAATC20800.1086896315316982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.014.5488581
TAGGACC10200.013.4217564
AACACCG550.003050627412.1005495
CGTGCGC550.00307281512.08884710
GACCTCG550.003073416712.0885317
CACCTTT8900.012.05564514
TCCAACG1354.7657522E-911.25380918
CCACCTT9050.011.12342913
ACCTTTT9350.011.06892615
GGACTTG3450.011.0123266
ATAGGAC3050.010.909763
TAGAAAT9700.010.7812214
CTTTTTC10950.010.66606717
TGTAGGA35900.010.6466432
GGACCTG19800.010.5534796
AGGACCT20550.010.5022845
TAGGACT4100.010.4345974
AGGACGT35200.010.37195
TCTATAC1659.1677066E-1010.3713573
TAGAACG1105.9929225E-610.3713564
Produced by FastQC (version 0.11.5)