FastQCFastQC Report
Thu 26 May 2016
SRR1512387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950644
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61080.31312735691392174No Hit
GTCCTAAAGTGTGTATTTCTCATTT46780.2398182343882328No Hit
GTCCTACAGTGGACATTTCTAAATT45450.2329999733421373No Hit
CTGTAGGACGTGGAATATGGCAAGA42630.21854320931958882No Hit
GGTATCAACGCAGAGTACTTTTTTT40170.2059319896403444No Hit
CTTTAGGACGTGAAATATGGCGAGG40110.20562439891646042No Hit
TATCAACGCAGAGTACTTTTTTTTT38240.19603782135540876No Hit
GTCCTACAGTGTGCATTTCTCATTT26040.13349437416566018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.013.3599721
TAGGACC9050.012.9959044
GTGTAGG3050.012.9505071
TCCAACG1451.0186341E-1011.760426518
GGCGAGG13050.011.47388719
CCAACGA1251.8997525E-811.3722219
AGGACCT18250.011.3302195
CTAATAC1950.011.1876243
GTCCTAC27050.011.0611931
ACCTTTT7450.010.95241815
TAGAAAT8700.010.9022024
AGGACGG1053.5325938E-610.8401825
CTAGGAC3500.010.8401813
GACGTGA16250.010.7987687
GGACCTG18000.010.6971516
TGGCGAG28900.010.6865718
ATCCCGT803.8557276E-410.66200610
TAATACT2600.010.5793284
GTGGTAT4250.010.455631
GTATTAG1801.9463187E-1010.423361
Produced by FastQC (version 0.11.5)