FastQCFastQC Report
Thu 26 May 2016
SRR1512387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950644
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68160.3494230623322349No Hit
CTGTAGGACGTGGAATATGGCAAGA50270.2577097614941527No Hit
GTCCTAAAGTGTGTATTTCTCATTT50010.256376868357322No Hit
TATCAACGCAGAGTACTTTTTTTTT47590.24397070916066693No Hit
GTCCTACAGTGGACATTTCTAAATT47480.24340679283354627No Hit
CTTTAGGACGTGAAATATGGCGAGG46790.23986949950888015No Hit
GGTATCAACGCAGAGTACTTTTTTT46130.23648600154615604No Hit
GTCCTACAGTGTGCATTTCTCATTT29090.14913023596309732No Hit
CTGAAGGACCTGGAATATGGCGAGA23520.12057556376253176No Hit
CTGTAGGACCTGGAATATGGCGAGA22540.11555158193909294No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21780.11165543276989548No Hit
ATTTAGAAATGTCCACTGTAGGACG20870.106990306790988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG400.00525482514.2601644
GGTATCA14600.013.9449671
GGCGAGG12550.011.57626619
GGCGTGC752.0774028E-411.3981848
GTACTAG855.2457708E-511.1927731
TAGGACC8000.011.1704624
CCTACCG600.005845464311.0912383
CTTAGAC1053.4375935E-610.8648873
ACGGGAA803.7771557E-410.6857988
CTTAAGC1355.6294084E-810.5630853
GTATAAC1909.094947E-1210.5153151
AGTCGTC2900.010.481098
GGACGGG1002.4034529E-510.4483366
TTTAGAA9100.010.3420052
AGGACCT16850.010.3248665
GTATAAT2500.010.2749651
TGTAGGA28500.010.2067512
CGGTTTC1409.730866E-810.17564513
TGGCGAG28650.010.1418618
TTACACG750.00263247310.140564
Produced by FastQC (version 0.11.5)