FastQCFastQC Report
Thu 26 May 2016
SRR1512386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257817
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67170.29749975308007687No Hit
GTCCTACAGTGGACATTTCTAAATT66930.2964367794201213No Hit
CTGTAGGACGTGGAATATGGCAAGA61600.27282990605527374No Hit
GTCCTAAAGTGTGTATTTCTCATTT54500.24138360194825356No Hit
CTTTAGGACGTGAAATATGGCGAGG46510.20599543718556462No Hit
GGTATCAACGCAGAGTACTTTTTTT41320.18300863178902452No Hit
GTCCTACAGTGTGCATTTCTCATTT37700.16697544575136072No Hit
TATCAACGCAGAGTACTTTTTTTTT37290.16515953241560322No Hit
CTGTAGGACCTGGAATATGGCGAGA26010.11519977039768944No Hit
CTGAAGGACCTGGAATATGGCGAGA23200.10275412046237584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.013.6990891
TAGGACC11400.012.9768964
CGTATCA801.9357347E-512.3392541
CCAACGA854.056932E-612.26352919
GTGTAGG5000.011.8456841
TCCAACG1251.9092113E-811.36859918
GGCGAGG14450.011.34540319
GTCCTAC34250.011.2980731
GCGTTGA700.001082865511.2816041
TGTAGGA37050.011.0313972
TCCTACA39100.011.0108422
AGGACGT36250.010.9090975
GGACCTG20550.010.8910726
TTAGGAC20300.010.88456253
GTATTAA2550.010.8391891
AGGACCT21650.010.8193175
GTAGGAC36850.010.70553
GGACGTG35700.010.6789446
GTATAGA3700.010.6717871
GACGTGG18000.010.5899527
Produced by FastQC (version 0.11.5)