FastQCFastQC Report
Thu 26 May 2016
SRR1512386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257817
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75060.33244501215111766No Hit
CTGTAGGACGTGGAATATGGCAAGA74510.33000903084705274No Hit
GTCCTACAGTGGACATTTCTAAATT72250.31999936221580405No Hit
GTCCTAAAGTGTGTATTTCTCATTT60560.2682236868621327No Hit
CTTTAGGACGTGAAATATGGCGAGG56350.24957735724374475No Hit
GGTATCAACGCAGAGTACTTTTTTT50260.22260440062237108No Hit
TATCAACGCAGAGTACTTTTTTTTT48500.21480926044936324No Hit
GTCCTACAGTGTGCATTTCTCATTT42380.18770343212049517No Hit
CTGTAGGACCTGGAATATGGCGAGA31590.13991390799165743No Hit
CTGAAGGACCTGGAATATGGCGAGA29200.12932846196126613No Hit
ATTTAGAAATGTCCACTGTAGGACG24030.10643023770305565No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23040.10204547135573876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.005249922614.2623095
TAGGACC12550.014.1676064
GGTATCA14350.013.927731
AGGACCT22500.011.8324355
CGATAAG658.0453814E-411.68892712
GGACCTG21150.011.6307216
TGTCGAG1251.8475475E-811.39392818
CGCCCTA1701.2732926E-1111.17051716
GGCGAGG15100.011.12982919
TGTAGGA40700.010.8164612
ATGTCGA1605.493348E-1010.68180717
GTGTAGG4200.010.6502921
TCGAACT1252.2578388E-710.63433219
ACCTTTT7600.010.619575515
GTAGGAC39150.010.588963
TGTCCAC10450.010.54287210
CTGTAGG40250.010.5222131
TAGAAAT9050.010.5063054
ATAGGAC3350.010.50163
GTCCTAA17950.010.4981721
Produced by FastQC (version 0.11.5)