FastQCFastQC Report
Thu 26 May 2016
SRR1512385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084036
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53250.17266335412427092No Hit
GTCCTACAGTGGACATTTCTAAATT40080.12995957245635265No Hit
CTGTAGGACGTGGAATATGGCAAGA35090.11377947598536463No Hit
GGTATCAACGCAGAGTACTTTTTTT32860.10654869139011347No Hit
GTCCTAAAGTGTGTATTTCTCATTT32380.10499228932476794No Hit
TATCAACGCAGAGTACTTTTTTTTT30890.10016095791359114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8900.013.533414
GGTATCA12750.011.8454051
GTAGGAC23250.011.1361043
TGTAGGA25950.011.0005812
TGGCGAG20200.010.97554918
CCCGTGT951.4393809E-510.9447588
GTATCAA28050.010.768551
GGCGAGG9100.010.72646619
GTCCTAC19900.010.7144371
AGGACCT15850.010.5311785
CTGTAGG23400.010.4195691
GTCCTAA10700.010.332441
GGCGAGA12850.010.32489819
AGGACGT20500.010.2242625
ATAGGAC4100.010.1779993
CGATACT750.002686018810.1163144
GACCCGT856.699565E-410.0419296
TCCTACA24000.010.0371162
TAGGACG22100.09.9990154
TATGGCG22200.09.95949616
Produced by FastQC (version 0.11.5)