FastQCFastQC Report
Thu 26 May 2016
SRR1512385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084036
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66080.21426468432923612No Hit
GGTATCAACGCAGAGTACTTTTTTT43040.13955738519265015No Hit
TATCAACGCAGAGTACTTTTTTTTT42870.13900615946117362No Hit
CTGTAGGACGTGGAATATGGCAAGA41980.13612033063167875No Hit
GTCCTACAGTGGACATTTCTAAATT41440.134369378308165No Hit
GTCCTAAAGTGTGTATTTCTCATTT34100.11056939672558945No Hit
CTTTAGGACGTGAAATATGGCGAGG31470.10204161040921701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.013.3698781
TAGGACC9450.011.6699264
ACCGATA600.0058848711.0815648
GTAGGAC22350.011.0170193
TGTAGGA25400.010.9294432
ACGCAAA700.001494112110.8554111
AGGACGT21050.010.794135
GGACGTG20800.010.7314616
TAGGACG22300.010.4875044
GTCCTAC23800.010.4338351
CTGTAGG25900.010.3960191
TGGCGAG21750.010.39020318
GACGTGG10750.010.3378847
GGCGAGG10600.010.12229419
CCATGCG1054.1149826E-59.9507929
ATGGCGA23800.09.93410517
ACGTGAA13200.09.8582748
GACGTGA11600.09.8260177
TATGGCG23400.09.81987416
CGTGAAA13300.09.7127359
Produced by FastQC (version 0.11.5)