FastQCFastQC Report
Thu 26 May 2016
SRR1512384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133694
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77580.3635947797575472No Hit
GTCCTACAGTGGACATTTCTAAATT64180.300792897200817No Hit
CTGTAGGACGTGGAATATGGCAAGA59190.2774062260099152No Hit
GTCCTAAAGTGTGTATTTCTCATTT56260.2636741725851973No Hit
CTTTAGGACGTGAAATATGGCGAGG47770.22388402460708987No Hit
GGTATCAACGCAGAGTACTTTTTTT46600.2184005766525097No Hit
TATCAACGCAGAGTACTTTTTTTTT44460.2083710222740468No Hit
GTCCTACAGTGTGCATTTCTCATTT30250.1417729065179918No Hit
CTGAAGGACCTGGAATATGGCGAGA26520.12429148697048406No Hit
GTACTGGTTCACTATCGGTCAGTCA23860.11182484461220775No Hit
GTCCTTCAGTGTGCATTTCTCATTT23030.10793487725981325No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22640.1061070612749532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.014.5690461
TAGGACC11050.013.1308384
CCACCTT8250.012.53310613
AGAACCG550.00310958112.0697595
CCGTACA1501.4551915E-1112.02021917
CTAGGAC3250.011.9636533
TTGCGAG802.9518947E-511.8413918
ATTGCGA907.5551834E-611.59845517
GGCGAGG14650.011.3830819
CACCTTT8900.011.3020114
TTCCACC9150.011.29341911
ATAGGAC3200.011.2615173
ACCTTTT8950.011.23886815
GCGCCAC855.404945E-511.16007813
TCAATAC1701.2732926E-1111.1569213
TCGTAGG600.005953928511.0639472
GGACCTG21100.010.876426
TAGGACT4450.010.8685974
TGCGAGA700.001524780410.8287119
AGGACCT22450.010.81401355
Produced by FastQC (version 0.11.5)