FastQCFastQC Report
Thu 26 May 2016
SRR1512384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512384_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133694
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT92530.4336610591771829No Hit
CTGTAGGACGTGGAATATGGCAAGA71020.33284997755067036No Hit
GTCCTACAGTGGACATTTCTAAATT68290.32005526565664993No Hit
GTCCTAAAGTGTGTATTTCTCATTT60770.28481122410242515No Hit
GGTATCAACGCAGAGTACTTTTTTT60310.282655338581821No Hit
TATCAACGCAGAGTACTTTTTTTTT60180.28204606658686765No Hit
CTTTAGGACGTGAAATATGGCGAGG59500.27885910538249625No Hit
CTGAAGGACCTGGAATATGGCGAGA33930.15901999068282519No Hit
GTCCTACAGTGTGCATTTCTCATTT32350.1516149925903152No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28630.13418044011934233No Hit
GTACTGGTTCACTATCGGTCAGTCA25800.12091705745997318No Hit
ATTTAGAAATGTCCACTGTAGGACG24250.1136526605970678No Hit
CTGTAGGACCTGGAATATGGCGAGA24180.11332459106132368No Hit
GTCCTTCAGTGTGCATTTCTCATTT23960.11229351537755648No Hit
TTTCTAAATTTTCCACCTTTTTCAG23210.10877848463744098No Hit
GAATATGGCAAGAAAACTGAAAATC22490.1054040551269301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14600.013.5552731
TAGGACC9350.012.5055834
TCCAACG1650.012.08500318
CGGTTTC1251.8409992E-811.39656913
GCGTGCG855.3334887E-511.1746789
GCGCCAC855.341135E-511.17310613
CCACCTT9200.011.14881713
CCAGCGG600.00588389511.0815568
GCCGGTT1303.275636E-810.9597811
GTATCAA33150.010.8207071
TGTAGGA34750.010.7514882
GATATAC6300.010.7229831
ACCTTTT9100.010.64389915
GGCGTGC1355.7032594E-810.5538648
GTGCGCC909.533456E-510.55386311
GTAGGAC34500.010.4706813
GACGTGG18200.010.4378857
GTATAGC3200.010.4067681
TAGGACA7500.010.3935284
AAGGCGT1106.0590537E-610.3619756
Produced by FastQC (version 0.11.5)