FastQCFastQC Report
Thu 26 May 2016
SRR1512383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137760
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63730.2981157847466507No Hit
CTGTAGGACGTGGAATATGGCAAGA58510.27369770226779433No Hit
GTCCTAAAGTGTGTATTTCTCATTT56250.26312588878077986No Hit
GTATCAACGCAGAGTACTTTTTTTT54200.2535364119452137No Hit
CTTTAGGACGTGAAATATGGCGAGG43750.2046534690517177No Hit
GGTATCAACGCAGAGTACTTTTTTT34770.1626468827183594No Hit
GTCCTACAGTGTGCATTTCTCATTT34330.16058865354389643No Hit
TATCAACGCAGAGTACTTTTTTTTT31210.1459939375795225No Hit
CTGAAGGACCTGGAATATGGCGAGA25050.11717872913704064No Hit
CTGTAGGACCTGGAATATGGCGAGA23250.10875870069605569No Hit
GTCCTTCAGTGTGCATTTCTCATTT22210.10389379537459771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10250.014.6205774
GGTATCA10800.012.4273611
GTCTTAC1900.011.9464251
ATAGGAC3100.011.9325753
GGCGAGG13950.011.8208519
AGGACCT21850.011.6336135
GGACCTG21200.011.4984356
TGTAGGA35900.011.4399652
GCGAACG752.1063589E-411.38130513
GTGTAGG4450.011.3102951
GTAGGAC34650.011.27782253
CTGTAGG32000.011.1332241
GTATTAA2400.011.1023851
AGGACGT34300.011.0334175
GGACGTG33500.010.929096
CCTAGAC1752.1827873E-1110.83984853
AGAACCG700.001511761910.83984855
TGCGATA1053.566438E-610.83169110
TCCAACG1053.5815356E-610.82787118
TTAGGAC19700.010.7848233
Produced by FastQC (version 0.11.5)