FastQCFastQC Report
Thu 26 May 2016
SRR1512383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137760
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69360.3244517625926203No Hit
GTCCTACAGTGGACATTTCTAAATT69240.3238904273632213No Hit
CTGTAGGACGTGGAATATGGCAAGA68670.3212240850235761No Hit
GTCCTAAAGTGTGTATTTCTCATTT57650.26967479979043485No Hit
CTTTAGGACGTGAAATATGGCGAGG51610.241420926577352No Hit
GGTATCAACGCAGAGTACTTTTTTT45760.2140558341441509No Hit
TATCAACGCAGAGTACTTTTTTTTT43990.20577613951051568No Hit
GTCCTACAGTGTGCATTTCTCATTT36800.1721428036823591No Hit
CTGAAGGACCTGGAATATGGCGAGA28590.13373811840431105No Hit
CTGTAGGACCTGGAATATGGCGAGA27410.1282183219818876No Hit
GTCCTTCAGTGTGCATTTCTCATTT24090.11268804730184866No Hit
ATTTAGAAATGTCCACTGTAGGACG23010.10763603023725771No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21690.10146134271386872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13250.014.2917241
TAGGACC10050.013.6225124
TAAGACG701.08338965E-412.2237714
TAGGACT3750.012.1694434
ATTGCGA658.063225E-411.68578717
GTGGTAT3450.011.3086871
TGTAGGA36050.011.2611672
GGCGAGG13800.011.14595519
CGCCCTA1803.6379788E-1211.07715216
CTAGGAC3050.010.9101073
GTAGGAC36300.010.8169353
CTGTAGG35100.010.790051
TGTCGAG1158.88409E-710.73314118
GCACCGT803.7766894E-410.6860396
AGGACCT19700.010.6658875
GTCCTAC31550.010.6468911
AGGACGT35050.010.6333235
GTCTTAC1803.45608E-1110.5731631
AATGTCC7650.010.5541128
GGACGTG35250.010.5361486
Produced by FastQC (version 0.11.5)