FastQCFastQC Report
Thu 26 May 2016
SRR1512382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110992
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67250.3185706056678566No Hit
GTCCTACAGTGGACATTTCTAAATT63110.29895897284309936No Hit
GTCCTAAAGTGTGTATTTCTCATTT59900.28375285173984555No Hit
CTGTAGGACGTGGAATATGGCAAGA58560.2774051251733782No Hit
CTTTAGGACGTGAAATATGGCGAGG50950.24135572280709733No Hit
GGTATCAACGCAGAGTACTTTTTTT42510.2013745196571091No Hit
TATCAACGCAGAGTACTTTTTTTTT37760.17887325011179578No Hit
GTCCTACAGTGTGCATTTCTCATTT32910.15589826962868641No Hit
CTGAAGGACCTGGAATATGGCGAGA27070.12823355086139596No Hit
CTGTAGGACCTGGAATATGGCGAGA23610.11184315241365198No Hit
ATTTAGAAATGTCCACTGTAGGACG23260.11018516413136571No Hit
GTCCTTCAGTGTGCATTTCTCATTT23230.11004305085002691No Hit
TTTCTAAATTTTCCACCTTTTTCAG22000.10421640631513526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12250.014.3324491
TATTCCG400.005339725414.2258255
TCGCATT552.0068616E-413.78029318
TAGGACC10650.013.7137844
CGCATTT905.5781493E-712.63434219
CCAACGA1207.712515E-1012.63434219
AGGACCT20550.011.8144735
TCCAACG1850.011.77842718
AATGTCC9150.011.7783858
GGCGAGG16100.011.59455919
GGACCTG20500.011.4271966
CGCGCCT1001.9747986E-611.37849112
AAATGTC8850.011.3597697
CCACCTT9700.011.14786613
TTCGCAT600.005929430511.07006917
CGGTTTC1803.6379788E-1211.06637713
ATGTCCA10100.011.0506949
TGTCCAC10400.011.03045510
GAAATGT9350.010.9549066
GTGTAGG4150.010.9331741
Produced by FastQC (version 0.11.5)