FastQCFastQC Report
Thu 26 May 2016
SRR1512382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2110992
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83430.3952170354032607No Hit
CTGTAGGACGTGGAATATGGCAAGA69840.3308397189567748No Hit
GTCCTACAGTGGACATTTCTAAATT67190.318286379105179No Hit
GTCCTAAAGTGTGTATTTCTCATTT64140.3038381955023989No Hit
CTTTAGGACGTGAAATATGGCGAGG59730.28294754314559223No Hit
GGTATCAACGCAGAGTACTTTTTTT57400.2719100782949438No Hit
TATCAACGCAGAGTACTTTTTTTTT55210.2615358087572099No Hit
GTCCTACAGTGTGCATTTCTCATTT35970.17039382432524613No Hit
CTGAAGGACCTGGAATATGGCGAGA33240.15746151572341344No Hit
CTGTAGGACCTGGAATATGGCGAGA27600.13074421883171514No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27390.1297494258623434No Hit
ATTTAGAAATGTCCACTGTAGGACG27090.12832829304895518No Hit
TTTCTAAATTTTCCACCTTTTTCAG25680.1216489688260306No Hit
GTCCTTCAGTGTGCATTTCTCATTT23790.11269583210168489No Hit
GAATATGGCAAGAAAACTGAAAATC23550.11155892585097432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1550.014.089745518
GGTATCA14250.013.7574421
GGCGAGT500.001505505613.29336819
TAGGACC11550.012.5948474
CCAACGA1001.4527723E-712.34384219
GGCGAGG16450.011.83300219
ACGATGC600.005887269511.0806938
ATCGCCG600.005898699611.07780619
GGACCTG22650.011.0707356
AGGACCT23950.011.0365425
AATGTCC9900.010.9367898
GTGGTAT4600.010.7579831
ACGGTAC1158.7266926E-710.7480323
TGTCCAC10350.010.73657310
GCGCCAC1158.863608E-710.73504613
TGTAGGA39000.010.5079182
TAGAAAT10650.010.3559944
CTGTAGG37550.010.3150141
TGGCGAG38500.010.30914218
AGGACCG1201.5076203E-610.3004415
Produced by FastQC (version 0.11.5)